| 引用本文： |
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姚军,张燕玲,林荣.苦丁茶的组织培养和快速繁殖研究[J].广西科学院学报,1996,(Z1):30-34. [点击复制]
- Yao Jun,Zhang Yanling,Lin Rong.Studies on the Tissue Culture and Rapid Propagation of Ilex Kudingcha[J].Journal of Guangxi Academy of Sciences,1996,(Z1):30-34. [点击复制]
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| 摘要: |
| 用苦丁茶当年生枝条的顶芽和侧芽作外植体,培养在附加了各种浓度BA的MS培养基上,当BA浓度为3.0mg/L时诱导芽形成及增殖效果最佳,GA2.0+NAA0.2mg/L和BA配合使用有利于壮苗;在1/2MS培养基中,附加NAA0.5mg/L,生根效果最佳。试管苗高2cm~3cm、根数2~3条时开盖炼苗2d~3d移入培养土中,试管苗移栽成活率高。 |
| 关键词: 苦丁茶 细胞分裂素 快速繁殖 |
| DOI： |
| 投稿时间：1996-04-15 |
| 基金项目: |
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| Studies on the Tissue Culture and Rapid Propagation of Ilex Kudingcha |
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Yao Jun, Zhang Yanling, Lin Rong
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| (Guangxi Inwtitute of Botany, Guilin, Guangxi, 541006) |
| Abstract: |
| The tissue culture of Ilex kudingcha with the tip of shoots and lateral bud as explants was experimented in MS medium with addition of different contentration of BA. The results show that sprouting of bud and multiplication of adventitious buds was best in MS medium with the addition of BA 3.0 mg/L; andvigorous and healthy bud was best on MS medium with the addition of BA 3.0+GA2.0+NAA 0.2 mg/I.; and the growth of roots was best on 1/2 MS medium with the addition of NAA 0.5 mg/L. Transplantation from test-tube, when the plantlets are 2 cm~3 cm high and have 2~3 roots and after they stayed 2 d~3d without cap of test-tube gave the highest percentage of survival. |
| Key words: kudingcha cytokinin rapid propagation |
