| 引用本文： |
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王爱民,苏琼,阎冰,陈晓汉,刘汀.马氏珠母贝外套膜组织培养的条件和方法初探[J].广西科学院学报,1995,(Z1):17-22. [点击复制]
- Wang Aimin,Su Qiong,Yan Bing,Chen Xiaohan,Liu Ting.A Preliminary Study on the Suitable Conditions and Methods of the Mantle Tissue Culture of the Pearl Oyster Pinctada martensii[J].Journal of Guangxi Academy of Sciences,1995,(Z1):17-22. [点击复制]
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| 摘要: |
| 经过反复试验,筛选出适宜于马氏珠母贝外套组织培养的平衡盐溶液(改进的MMBSS)、基本培养基;确立了防止污染的组织净化方法,并筛选出一种能促进细胞生长的贴壁物质(SM)。其主要培养方法为:用合抗生素的改进MMBSS(青霉素2000U/ml,链霉素2500U/ml)洗涤10次,再用不含抗生素的改进MMBSS清洗5次,将组织切成3mm×3mm的小块,贴于含0.5% SM的培养瓶底,加入改进的贝培养基,在20℃,pH6.8条件下培养。经这种条件和方法培养的马氏珠母贝外套膜组织,4小时细胞开始游离,3天游离出来的细胞铺满瓶底,4天细胞分泌珍珠质.培养7天大量的成纤维细胞出现,细胞培养能持续数十天。 |
| 关键词: 马氏珠母贝 外套膜 组织培养 条件 方法 |
| DOI： |
| 投稿时间：1995-06-19 |
| 基金项目:国家科委地方重大科技攻关 |
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| A Preliminary Study on the Suitable Conditions and Methods of the Mantle Tissue Culture of the Pearl Oyster Pinctada martensii |
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Wang Aimin1, Su Qiong1, Yan Bing1, Chen Xiaohan1, Liu Ting2
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| (1.Guangxi Institute of Oceanography, East Nanzhu Road, Beihai, 536000;2.Life Science College, Wuhan University, Wuhan, 430072) |
| Abstract: |
| Balance salt solution (BSS) based on the MMBSS and culture medium for the mantle tissue culture of the pearloyster,Pinctada martensii were selected affer repeated experiments.A decontaminating method for the culture was established,and a sticking material(SM) promoting the culture cells growth was also screened.The culture method for the mantle tissue of the pearl oyster is as follows.The strips of mantle tissue were rinsed 10 times with improved MMBSS containing 2000U/ml penicillin and 2500U/ml streptomysin,and were washed 5 times with improved MMBSS without antibiotic,and then cut out into 3mm×3mm pieces.The tissue fragments were inoculated in the culture flasks covered with 0.5% SM,and 3 ml culture medium was added to each culture.The culturs were incubated at 20℃ and pH 6.8.Four hours after initiation of the culture the epithelial-like cells began to migrate out from the explants. After 3ds culture,numerous epithelial-like cells were massed on the flask. By 4ds the pearl materials were secreted by the epithelial-like cells.After7ds culture, fibroblast-like cells also began outward migration from the explants.The culture of mantle tissue can be maintained several ten days. |
| Key words: Pinctada martensii Pearl oyster mantle tissue culture condition and method |
