| 引用本文： |
-
梁冰,覃兰芳,赖茂祥,刘布鸣,刘华钢,赖克道.高效液相色谱法测定广西壮药毛郁金中姜黄素的含量[J].广西科学,2012,19(2):149-151. [点击复制]
- LIANG Bing,QIN Lan-fang,LAI Mao-xiang,LIU Bu-ming,LIU Hua-gang,LAI Ke-dao.Determination of Curcumin in Rhizome of Curcuma aromatica by HPLC[J].Guangxi Sciences,2012,19(2):149-151. [点击复制]
|
|
| |
|
|
| 本文已被：浏览 605次 下载 427次 |
码上扫一扫！ |
| 高效液相色谱法测定广西壮药毛郁金中姜黄素的含量 |
|
梁冰1, 覃兰芳1, 赖茂祥1,2, 刘布鸣1,2, 刘华钢3, 赖克道1
|
|
|
| (1.广西中医药研究院, 广西南宁 530022;2.广西中药质量标准研究重点实验室, 广西南宁 530022;3.广西医科大学, 广西南宁 530021) |
|
| 摘要: |
| 采用Ultimate(XB-C18)色谱柱,以乙腈-4%冰乙酸(48:52)为流动相,流速1.0 ml/min,检测波长430nm,柱温为室温,建立高效液相色谱法(HPLC)测定广西壮药材毛郁金中姜黄素含量的方法。结果表明,姜黄素在0.00992~0.08928μg范围内的峰面积与其浓度呈良好线性关系(r=0.9995),平均回收率为101.28%,RSD为1.67%(n=6)。本方法准确,快速,重现性好,可以作为广西壮药材毛郁金的含量测定方法。 |
| 关键词: 含量测定 姜黄素 毛郁金 HPLC |
| DOI： |
| 投稿时间：2012-02-07 |
| 基金项目:广西创新能力建设项目(编号:11-114-14D),广西壮药质量标准研究项目(编号:MZY20100042),广西自然科学基金项目(2010GXNSFD013044)资助。 |
|
| Determination of Curcumin in Rhizome of Curcuma aromatica by HPLC |
|
LIANG Bing1, QIN Lan-fang1, LAI Mao-xiang1,2, LIU Bu-ming1,2, LIU Hua-gang3, LAI Ke-dao1
|
| (1.Guangxi Institute of Traditional Medical and Pharmaceutical Science, Nanning, Guangxi, 530022, China;2.Guangxi Key Laboratory of Tradtitonal Chinese Medicine Quality Stardands, Nanning, Guangxi, 530022, China;3.Guangxi Medical University, Nanning, Guangxi, 530021, China) |
| Abstract: |
| A HPLC method was established to determine Curcumin in rhizome of Curcuma aromatica. Ultimate XB-C18 column was used as chromatographic column. The mobile phase was acetonitrile-4% acetic acid solution (48:52). The flow rate was 1.0 ml·min-1 and the detection wavelength was set at 430nm. The column temperature was maintained at room temperature. The calibration curve of Curcumin was in good linearity over the range of 0.00992~0.08928(μg). The average recovery was 101.28%, with RSD of 1.67%(n=6). This method is convenient, sensitive, reliable and suitable for determination of Curcumin in rhizome of Curcuma aromatica. |
| Key words: content determination curcumin Curcuma aromatica HPLC |
|
|
|
|
|
