| 引用本文： |
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冼康华,唐健民,苏江,周龙武,丁涛,韦霄,邓敏敏.两种树龄元宝山冷杉根际与非根际土壤微生物群落多样性[J].广西科学,2025,32(6):1095-1106. [点击复制]
- XIAN Kanghua,TANG Jianmin,SU Jiang,ZHOU Longwu,DING Tao,WEI Xiao,DENG Minmin.Microbial Diversity in Rhizosphere and Non-rhizosphere Soils of Abies yuanbaoshanensis of Two Ages[J].Guangxi Sciences,2025,32(6):1095-1106. [点击复制]
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| 摘要: |
| 为探究元宝山冷杉生长发育与土壤微环境之间的关系，本研究采用高通量测序技术对幼龄和老龄阶段元宝山冷杉根际与非根际土壤细菌16S rRNA和真菌ITS区进行测序分析。结果表明：①幼龄树根际土壤中细菌和真菌的丰富度与多样性均显著高于老龄树。幼龄树根际与非根际土壤细菌的丰富度与多样性差异不显著，而老龄树根际土壤细菌的丰富度与多样性均显著低于非根际土壤。幼龄树根际土壤真菌的丰富度显著高于非根际土壤，而老龄树根际土壤真菌的丰富度则显著低于非根际土壤，但幼龄树和老龄树根际与非根际土壤中真菌的多样性差异均不显著。②元宝山冷杉根际与非根际土壤中主要优势细菌门为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)和放线菌门(Actinobacteriota)；主要优势真菌门为担子菌门(Basidiomycota)和子囊菌门(Ascomycota)。在纲水平上，主要优势细菌纲为α-变形菌纲(Alphaproteobacteria)和酸杆菌纲(Acidobacteriae)，主要优势真菌纲为伞菌纲(Agaricomycetes)和子囊菌门中纲级分类地位未知的类群(Unclassified_p_Ascomycota)。③主坐标分析(Principal Co-ordinates Analysis，PCoA)结果显示，幼龄树与老龄树土壤细菌和真菌群落组成差异显著；老龄树根际与非根际土壤中微生物群落组成的差异大于幼龄树。④线性判别分析效应大小(Linear Discriminant Analysis Effect Size, LEfSe)分析发现，元宝山冷杉非根际土壤细菌和真菌群落具有更高的特异性、丰富性和多样性，而元宝山冷杉的定植也使其根际土壤形成了特异性的细菌和真菌群落。土壤微生物群落组成随着元宝山冷杉生长发育而变化，老龄树对土壤微生物的影响大于幼龄树，导致土壤中细菌和真菌群落结构发生显著变化。 |
| 关键词: 元宝山冷杉|土壤|微生物群落|多样性|高通量测序 |
| DOI：10.13656/j.cnki.gxkx.20250815.001 |
| 投稿时间：2023-11-06修订日期：2024-01-04 |
| 基金项目:中国科学院“西部之光”人才培养计划项目(2022)，国家重点研发计划项目(2022YFF1300703)，国家自然科学基金项目(32160091，32060248)，广西林业科技推广示范项目(2023LYKJ03，〔2022〕GT23)，广西植物功能物质与资源持续利用重点实验室自主项目(ZRJJ2022-2)，桂林市创新平台和人才计划项目(20210102-3)，广西喀斯特植物保育与恢复生态学重点实验室项目(22-035-26)资助。 |
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| Microbial Diversity in Rhizosphere and Non-rhizosphere Soils of Abies yuanbaoshanensis of Two Ages |
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XIAN Kanghua1, TANG Jianmin1, SU Jiang1, ZHOU Longwu1, DING Tao1, WEI Xiao1, DENG Minmin2
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| (1.Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China;2.Rongshui Miao Autonomous County Yuanbaoshan National Nature Reserve Management Center, Liuzhou, Guangxi, 545300, China) |
| Abstract: |
| To explore the relationship of the growth and development of Abies yuanbaoshanensis with soil microenvironment, this study employed high-throughput sequencing to analyze the 16S rRNA gene of bacteria and ITS region of fungi in the rhizosphere and non-rhizosphere soils of young and old A.yuanbaoshanensis.The results are as follows: ①The abundance and diversity of bacteria and fungi in the rhizosphere soil of young trees were significantly higher than those of old trees.There was no significant difference in the abundance or diversity of bacteria between rhizosphere soil and non-rhizosphere soil of young trees, while the abundance and diversity of bacteria in rhizosphere soil were significantly lower than those in non-rhizosphere soil of old trees.The abundance of fungi in rhizosphere soil of young trees was significantly higher than that in non-rhizosphere soil, while this trend became opposite for old trees.The diversity of fungi in rhizosphere soil of both young trees and old trees was not significantly different from that in non-rhizosphere soil.②Proteobacteria, Acidobacteriota and Actinobacteriota were dominant bacterial phyla in rhizosphere and non-rhizosphere soils of A.yuanbaoshanensis.The dominant fungal phyla were Basidiomycota and Ascomycota.The dominant bacterial classes were Alphaproteobacteria and Acidobacteriae, while the dominant fungal classes were Agaricomycetes and Unclassified_p_Ascomycota.③Principal Co-ordinates Analysis (PCoA) showed that there were significant differences in soil bacterial and fungal community composition between young trees and old trees.The difference of microbial community composition between rhizosphere and non-rhizosphere soils of old trees was greater than that of young trees.④Linear Discriminant Analysis Effect Size (LEfSe) showed that the bacterial and fungal communities in the non-rhizosphere soil of A.yuanbaoshanensis had higher specificity, richness and diversity than those in the rhizosphere soil.The colonization of A.yuanbaoshanensis formed specific bacterial and fungal communities in the rhizosphere soil.In conclusion, the soil microbial community composition changed with the growth and development of A.yuanbaoshanensis.And the influence of old trees on soil microbial community was greater than that of young trees, which resulted in significant changes in the community structures of bacteria and fungi in soil. |
| Key words: Abies yuanbaoshanensis|soil|microbial community|diversity|high-throughput sequencing |
