| 摘要: |
| 为探讨等离子体活化水(Plasma-Activated Water,PAW)对金黄色葡萄球菌(Staphylococcus aureus)的灭活及其作用机制，本研究采用转录组学技术分析等离子体活化水处理后的金黄色葡萄球菌基因表达变化情况。结果表明，等离子体活化水能够显著灭活金黄色葡萄球菌，处理5 min后细菌存活数量降至检出限以下。转录组学分析结果显示，577个基因发生差异表达，其中317个基因上调，260个基因下调。基因本体论(Gene Ontology,GO)、蛋白直系同源簇(Clusters of Orthologous Groups of proteins，COG)、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)功能注释及富集分析结果显示，差异表达基因主要涉及翻译、氨基酸代谢、碳水化合物代谢和跨膜运输等生物过程。特别是与肠毒素相关的基因显著下调，表明等离子体活化水可能通过抑制细菌的毒力因子表达来减弱其致病性。同时，与蛋白质合成和核酸代谢相关的基因显著下调，表明等离子体活化水可能通过破坏细菌的蛋白质和核酸代谢途径，进一步抑制其生长和繁殖。此外，多个与氧化还原反应相关的基因显著上调，提示细菌通过激活氧化还原酶系统来应对等离子体产生的活性氧物种(Reactive Oxygen Species,ROS)和氧化应激。本研究为等离子体活化水的杀菌机制提供了新的见解，并为其在医疗和环境消毒等领域的应用奠定了理论基础。 |
| 关键词: 等离子体活化水 金黄色葡萄球菌 基因表达分析 灭菌机制 |
| DOI：10.13656/j.cnki.gxkx.20250521.016 |
| 投稿时间：2024-12-18修订日期：2025-01-05 |
| 基金项目:国家自然科学基金项目(51977174)资助。 |
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| Effect of Plasma-Activated Water Treatment on Gene Expression of Staphylococcus aureus |
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WANG Zhengyu1, GUO Li2
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| (1.SDU-ANU Joint Science College, Shandong University, Weihai, Shandong, 264209, China;2.Center for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China) |
| Abstract: |
| The inactivation effect and mechanism of Plasma-Activated Water (PAW) on Staphylococcus aureus were investigated.Transcriptomic analysis was performed to examine the gene expression changes in S.aureus after treatment with plasma-activated water.The results showed that plasma-activated water significantly inactivated S.aureus,reducing the survived bacterial count below the detection limit after treatment for 5 min.Transcriptomic analysis revealed 577 differentially expressed genes,including 317 genes with upregulated expression and 260 genes with downregulated expression.The Gene Ontology (GO),Clusters of Orthologous Groups of proteins (COG),and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation and enrichment analysis showed that the differentially expressed genes were primarily involved in biological processes such as translation,amino acid metabolism,carbohydrate metabolism,and transmembrane transport.Notably,genes related to enterotoxins were significantly downregulated,which suggested that plasma-activated water may reduce the pathogenicity of S.aureus by suppressing the expression of virulence factors.Furthermore,the genes associated with protein synthesis and nucleic acid metabolism were significantly downregulated,which indicated that plasma-activated water may disrupt bacterial protein and nucleic acid metabolism to inhibit bacterial growth and reproduction.Additionally,multiple genes related to redox reactions were significantly upregulated,indicating that bacteria counteracted oxidative stress by activating the redox enzyme system in response to Reactive Oxygen Species (ROS) and oxidative stress generated by plasma-activated water.The findings give new insights into the bactericidal mechanisms of plasma-activated water and lays a theoretical foundation for its application in medical disinfection and environmental sanitation. |
| Key words: plasma-activated water Staphylococcus aureus transcriptome analysis sterilization mechanism |
