| 引用本文： |
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胡颖,陈璐,江彩华,凌兰鑫,苏健.昂天莲叶提取物对糖苷酶的抑制作用及其化学成分研究[J].广西科学,2019,26(5):577-582. [点击复制]
- HU Ying,CHEN Lu,JIANG Caihua,LING Lanxin,SU Jian.The extracts from leaf of Ambroma augustum inhibit the activity of alpha-glucosidase and the chemical constitution study[J].Guangxi Sciences,2019,26(5):577-582. [点击复制]
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| 摘要: |
| 为研究昂天莲叶提取物对糖苷酶的抑制作用及其化学成分，本文采用系统溶剂提取法获得昂天莲叶不同极性的提取部位，并测试其对α-葡萄糖苷酶活性的抑制作用，然后用气相质谱法对昂天莲叶中的化学成分进行分析。实验结果发现，正己烷、氯仿、甲醇等提取部位对α-葡萄糖苷酶有较强的抑制作用，而不同的提取部位抑制曲线也有所不同；气质联用结果检识出23个高匹配度的化合物。表明昂天莲叶提取物对糖苷酶有抑制作用，气质联用检识出23个化学成分，其活性成分有待进一步确认。 |
| 关键词: 昂天莲叶 α-葡萄糖苷酶 化学成分 气质联用 糖尿病 |
| DOI：10.13656/j.cnki.gxkx.20191024.005 |
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| 基金项目:广西高校中药药理重点实验室项目（Jl700304），广西壮瑶药重点实验室项目（桂科基字〔2014〕32号），壮瑶药协同创新中心项目（桂教科研〔2013〕20号）和广西中药一流学科中药学（民族医药方向）项目（桂教科研〔2018〕12号）资助。 |
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| The extracts from leaf of Ambroma augustum inhibit the activity of alpha-glucosidase and the chemical constitution study |
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HU Ying1, CHEN Lu1, JIANG Caihua1, LING Lanxin2, SU Jian2
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| (1.College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China;2.Guangxi Scientific Research Center of Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China) |
| Abstract: |
| To study the inhibition of extracts from leaf of Ambroma augustum to alpha-glucosidase and the chemical constitution of the extract,systematic solvent extracts from leaf of Ambroma augustum were prepared to test the inhibition to alpha-glucosidase.A GC-MS method was used to analyze the chemical constitution.The results showed that the extraction sites of n-hexane,chloroform and methanol had strong inhibitory effects on α-glucosidase,and the inhibition curves of different extraction sites were also different.The results of GC-MS detected 23 compounds with high correlation.It indicated that extracts from the leaf of Ambroma augustum inhibit the activity of glucosidase,and 23 chemical components from the leaf were detected by GC-MS,whose active components were to be further confirmed. |
| Key words: Ambroma augustum alpha-glucosidase chemical components GC-MS diabetes |
