| 引用本文： |
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陈五九,曹鹏,宋国田,彭彦峰,吴凤礼,王钦宏.左旋多巴的合成研究新进展[J].广西科学,2018,25(3):274-278. [点击复制]
- CHEN Wujiu,CAO Peng,SONG Guotian,PENG Yanfeng,WU Fengli,WANG Qinhong.Advanced Research Progress in L-DOPA Synthesis[J].Guangxi Sciences,2018,25(3):274-278. [点击复制]
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| 摘要: |
| 左旋多巴(L-DOPA)是治疗帕金森病的最有效药物,在各国的临床中应用广泛。左旋多巴的制备方法包括化学合成、植物提取、酶催化以及微生物合成。化学合成法存在低转化率和环境污染问题。植物提取法不仅产量低,还受到资源限制。酶催化法使用包括酪氨酸酚解酶、酪氨酸酶及羟基苯乙酸3-羟化酶等催化苯类前体合成左旋多巴。随着合成生物学的发展,构建代谢工程微生物、发酵法生产左旋多巴是一种高效、环境友好的新方法,具有良好的应用前景。 |
| 关键词: 左旋多巴 不对称合成 植物提取 酶催化 代谢工程 |
| DOI：10.13656/j.cnki.gxkx.20180531.001 |
| 投稿时间：2018-04-29 |
| 基金项目:国家重点基础研究发展计划(973计划)项目(2011CBA00800)和天津市科技计划项目(14ZCZDSY00066)资助 |
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| Advanced Research Progress in L-DOPA Synthesis |
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CHEN Wujiu, CAO Peng, SONG Guotian, PENG Yanfeng, WU Fengli, WANG Qinhong
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| (CAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China) |
| Abstract: |
| L-DOPA is the most effective drug for Parkinson's disease and has been widely used in clinical treatment of various countries.The preparation methods of L-DOPA include chemical synthesis, plant extraction, enzyme catalysis, and microbial synthesis.However, Chemical synthesis has low conversion rate and environmental pollution problems.The plant extraction method not only has a low yield but is also subject to resource constraints.Enzymatic to L-DOPA has been catalyzed by tyrosine phenol lyase, tyrosinase and p-hydroxyphenylacetate 3-hydroxylase.The enzyme-catalyzed method uses catalyzed benzene precursors such as tyrosine phenolase, tyrosinase and p-hydroxyphenylacetate 3-hydroxylase to synthesize L-DOPA.With the development of synthetic biology, fermentation of L-DOPA by constructing metabolically engineered microorganisms would be a new high efficient and environment-friendly method, which demonstrates a promising process. |
| Key words: L-DOPA asymmetric synthesis plant extraction enzymatic metabolic engineering |
