| 引用本文： |
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苏青,雷沛霖,黄瑞松.壮药制剂武打将军酊质量标准研究[J].广西科学,2015,22(6):620-626. [点击复制]
- SU Qing,LEI Pei-lin,HUANG Rui-song.Study on Quality Standards of Wudajiangjun Tincture[J].Guangxi Sciences,2015,22(6):620-626. [点击复制]
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| 摘要: |
| [目的]为确保壮药制剂武打将军酊临床用药安全有效,建立其质量标准。[方法]采用薄层色谱法对武打将军酊处方中的虎杖、莪术、川芎、徐长卿、龙血竭、薄荷脑等药材进行定性鉴别;采用高效液相色谱法对酊剂中的虎杖苷、丹皮酚有效成分进行含量测定。[结果]建立上述6味药材的薄层色谱鉴别法,色谱图特征斑点分离清晰、集中。高效液相色谱法中虎杖苷对照品进样量在0.1~0.7 μg时,进样量与峰面积呈良好的线性关系;丹皮酚对照品进样量在0.052~0.364 μg时,进样量与峰面积呈良好的线性关系;虎杖苷平均回收率为100.98%,RSD=1.09%;丹皮酚平均回收率为99.62%,RSD=0.91%。[结论]本文建立的武打将军酊质量标准可有效控制壮药制剂武打将军酊的质量。 |
| 关键词: 武打将军酊 质量标准 TLC HPLC |
| DOI： |
| 投稿时间：2015-10-19修订日期：2015-11-27 |
| 基金项目:广西科学研究与技术开发计划项目(桂科重1355001-4),广西壮族自治区卫生厅中医药医院制剂类课题(GZYZ-10-27)和南宁市科学研究与技术开发计划项目(201102087C)资助。 |
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| Study on Quality Standards of Wudajiangjun Tincture |
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SU Qing, LEI Pei-lin, HUANG Rui-song
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| (Guangxi Academy of Minority Nationality Medicine and Pharmacology, Nanning, Guangxi, 530001, China) |
| Abstract: |
| [Objective] The quality standards for Wudajiangjun Tincture were established in order to guarantee its safety and effectivity in clinical treatment.[Methods] Thin layer chromatograms (TLC) method was used for qualitative identification of extracts from the tincture, including giant knotweed rhizome, zedoray rhizome, sichuan lovage rhizome, paniculate swallowwort root, Resina draconis, mentha-camphor and other medicinal materials qualitative.High performance liquid chromatography (HPLC) method was used to quantitatively determine active ingredients of the tincture such as polydatin and paeonol.[Results] Characteristics of TLC spots for the abovementioned 6 medicinal materials were clear separation and concentrated.In HPLC method, a good linear relationship between sample content and peak area was received when the reference substance of polydatin was ranged from 0.1 to 0.7 μg, and when paeonol concentration was within the range of 0.052 to 0.364 μg.[Conclusion] The scientific quality standards of Wudajiangjun Tincture are successfully established, which can be used to effectively control the product quality for Zhuang medicine preparations of Wudajiangjun Tincture. |
| Key words: Wudajiangjun Tincture quality standards TLC HPLC |
