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姚金娥,杨渊,孙海燕,唐林,巫繁菁,韦玮,黄慧学,高程海.小月柳珊瑚中生物碱类化学成分及其抗骨肉瘤细胞增殖活性研究[J].广西科学,2015,22(3):341-345. [点击复制]
- YAO Jin-e,YANG Yuan,SUN Hai-yan,TANG Lin,WU Fan-jing,WEI Wei,HUANG Hui-xue,GAO Cheng-hai.Chemical Compositions of Alkaloids from Marine Gorgonian Menella kanisa and Their activities of Anti-proliferation on Human Osteosarcoma Cell U2OS[J].Guangxi Sciences,2015,22(3):341-345. [点击复制]
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| 小月柳珊瑚中生物碱类化学成分及其抗骨肉瘤细胞增殖活性研究 |
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姚金娥1, 杨渊1, 孙海燕2, 唐林1, 巫繁菁1, 韦玮1, 黄慧学3, 高程海4
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| (1.广西中医骨伤科研究所, 广西南宁 530012;2.中国科学院三亚深海科学与工程研究所, 海南三亚 572000;3.广西中医药大学, 广西南宁 530001;4.广西科学院广西近海海洋环境科学重点实验室, 广西南宁 530007) |
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| 摘要: |
| [目的]研究小月柳珊瑚(Menella kanisa)中生物碱类化学成分及其抗骨肉瘤细胞增殖活性。[方法]采用多种分离方法和波谱分析方法,对小月柳珊瑚中生物碱类化学成分进行分离和结构鉴定。采用MTT法测试部分生物碱类化合物抗骨肉瘤细胞U2OS增殖活性。[结果]从小月柳珊瑚中分离出7个化合物,分别鉴定为6-(1'-purine-6',8'-dionyl) suberosanone (1)、1-(3'-carbonylbutyl) purine-6,8-dione (2)、腺嘌呤脱氧核苷(3)、次黄嘌呤(4)、7-甲基腺嘌呤(5)、胸腺嘧啶脱氧核苷(6)、尿嘧啶脱氧核苷(7)。化合物1和2对骨肉瘤细胞U2OS增殖显示出抑制活性,IC50值分别为13.3 μg/mL和55.0 μg/mL。[结论]化合物1~7均首次从小月柳珊瑚中分离得到,其中化合物1和2的抗骨肉瘤细胞活性也为首次报道。 |
| 关键词: 小月柳珊瑚 生物碱 结构鉴定 抗肿瘤细胞活性 |
| DOI： |
| 投稿时间：2015-02-26修订日期：2015-03-12 |
| 基金项目:广西科技攻关项目(桂科攻1140003B),三亚市院地科技合作项目(2013YD73),广西自然科学基金项目(2011GXNSFB018077)资助。 |
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| Chemical Compositions of Alkaloids from Marine Gorgonian Menella kanisa and Their activities of Anti-proliferation on Human Osteosarcoma Cell U2OS |
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YAO Jin-e1, YANG Yuan1, SUN Hai-yan2, TANG Lin1, WU Fan-jing1, WEI Wei1, HUANG Hui-xue3, GAO Cheng-hai4
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| (1.Guangxi Institute of Traditional Medical Traumatology & Orthopedics, Nanning, Guangxi, 530012, China;2.Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hainan, 572000, China;3.Guangxi University of Chinese Medicine, Nanning Guangxi, 530001, China;4.Guangxi Key Laboratory of Coastal Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objective] The alkaloids from marine gorgonian Menella kanisa and their activities of anti-proliferation on U2OS cancer cell were investigated.[Methods] The alkaloids were isolated and purified by column chromatography on silica gel, and their structures were identified by spectral analyses and compared with literatures.The cytotoxicity of compounds 1~2 toward human osteosarcoma cell U2OS cancer cell line was evaluated by MTT methods.[Results] Seven alkaloids were obtained from M.kanisa and characterized as 6-(1'-purine-6', 8'-dionyl)suberosanone (1), 1-(3'-carbonylbutyl)purine-6, 8-dione (2), adenine deoxyribonucleoside (3), hypoxanthine (4), 7-methyladenine (5), thymine deoxyribonucleoside (6) and uracil deoxyriboside (7).Compounds 1 and 2 showed moderate cytotoxicity against osteosarcoma U2OS cell line with IC50 of 13.3 and 55.0 μg/mL, respectively.[Conclusion] Compounds 1~7 were isolated from gorgonian M.kanisa for the first time.The antitumor activity of Compounds 1 and 2 against U2OS in vitro was also reported for the first time. |
| Key words: Menella kanisa alkaloid chemical identification anticancer |
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