| 引用本文： |
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冯家勋,赵帅.青霉纤维素酶基因的表达调控与重组表达研究进展[J].广西科学,2015,22(1):9-19,26. [点击复制]
- FENG Jia-xun,ZHAO Shuai.Advances in Regulation of Cellulase Gene Expression in Filamentous Fungi Penicillium and Recombinant Expression of Penicillium Cellulase Genes[J].Guangxi Sciences,2015,22(1):9-19,26. [点击复制]
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| 摘要: |
| 青霉属菌株可以分泌完整的纤维素酶系,尤其高产β-葡萄糖苷酶(β-glucosidase,BGL,EC 3.2.1.21),弥补了工业菌株里氏木霉(Trichoderma reesei)胞外β-葡萄糖苷酶活性低的不足,加上青霉菌株生长速度快等优点,使其产纤维素酶受到越来越多的关注。为了解决以木质纤维素为原料工业生产燃料乙醇所需纤维素酶量大、成本高等难题,深入研究青霉属纤维素酶基因的表达调控和重组表达非常重要。本文就青霉属纤维素酶基因资源、纤维素酶合成调控网络以及纤维素酶基因的重组表达等进行综述与展望。 |
| 关键词: 青霉 纤维素酶基因资源 纤维素酶合成调控网络 纤维酶基因重组表达 |
| DOI：10.13656/j.cnki.gxkx.20150126.004 |
| 投稿时间：2015-01-12 |
| 基金项目:国家自然科学基金项目(31260017),广西自然科学基金创新研究团队项目(2012GXNSFGA060005),广西八桂学者计划项目(2011A001)和广西科技攻关与新产品试制项目(桂科攻11107008-5)资助。 |
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| Advances in Regulation of Cellulase Gene Expression in Filamentous Fungi Penicillium and Recombinant Expression of Penicillium Cellulase Genes |
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FENG Jia-xun, ZHAO Shuai
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| (State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China) |
| Abstract: |
| Besides fast growth of Penicillium strains as compared to Trichoderma reesei, Penicillium strains can secret intact cellulase system, especially producing highly β-glucosidase, which can compensate the low activity of extracellular β-glucosidase of T.reesei.Therefore, the cellulases from Penicillium have attracted the most attention in recent years.To overcome the bottleneck of vast demand and high cost of the cellulases in industrially producing fuel ethanol from lignocelluloses, it is very important to deeply study the regulation of cellulase gene expression in Penicillium and recombinant expression of Penicillium cellulase genes.This paper reviews the aspects of cellulase gene resources from Penicillium, regulatory network of cellulase synthesis in Penicillium and high recombinant expression of Penicillium cellulase genes. |
| Key words: Penicillium cellulase gene resource regulatory network of cellulase synthesis recombinant expression of cellulase genes |
