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吴兆鹏,曾练强,黄曾慰,常国炜,梁达奉,李雨虹.重组毕赤酵母发酵甘油制备α-葡聚糖酶的研究[J].广西科学,2014,21(6):624-628. [点击复制]
- WU Zhao-peng,ZENG Lian-qiang,HUANG Zeng-wei,CHANG Guo-wei,LIANG Da-feng,LI Yu-hong.Study on the Fermentation Conditions for α-Glucanase Production by Recombinant Pichia pastoris Using Glycerol as Carbon Source[J].Guangxi Sciences,2014,21(6):624-628. [点击复制]
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| 重组毕赤酵母发酵甘油制备α-葡聚糖酶的研究 |
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吴兆鹏1, 曾练强1, 黄曾慰1, 常国炜1, 梁达奉1,2, 李雨虹1
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| (1.广州甘蔗糖业研究所, 广东省甘蔗改良与生物炼制重点实验室, 广东广州 510316;2.广西农垦糖业集团股份有限公司, 广西糖业研发中心, 广西南宁 530002) |
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| 摘要: |
| [目的]考察甘油对组成型毕赤酵母(Pichia pastoris)生长和α-葡聚糖酶表达量的影响。[方法]采用单因素实验法考察初始发酵培养基中甘油浓度、补料阶段甘油残留和通气量等对α-葡聚糖酶表达量的影响。[结果]发酵培养基中初始甘油浓度从50 g/L提高到150 g/L,菌体生长和α-葡聚糖酶表达量均未受到影响,继续提高到200 g/L时,α-葡聚糖酶表达量明显下降。补料过程甘油残留在0~5 g/L,菌体生长和α-葡聚糖酶表达量最佳,当甘油残留较多时,菌体生长和α-葡聚糖酶的表达量均受到影响。提高通气量有利于增加α-葡聚糖酶的表达量,发酵78 h为宜,在此条件下α-葡聚糖酶酶活力达1763 U。[结论]该工艺优化了以甘油作为碳源制备α-葡聚糖酶的发酵条件,提高了α-葡聚糖酶酶活力,为大规模生产α-葡聚糖酶奠定了基础。 |
| 关键词: 毕赤酵母 α-葡聚糖酶 甘油 发酵条件 |
| DOI： |
| 投稿时间：2014-10-10修订日期：2014-10-20 |
| 基金项目:现代农业产业技术体系建设专项项目(编号CARS-20-4-5)和八桂学者建设工程专项经费项目资助。 |
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| Study on the Fermentation Conditions for α-Glucanase Production by Recombinant Pichia pastoris Using Glycerol as Carbon Source |
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WU Zhao-peng1, ZENG Lian-qiang1, HUANG Zeng-wei1, CHANG Guo-wei1, LIANG Da-feng1,2, LI Yu-hong1
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| (1.Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, Guangdong, 510316, China;2.Guangxi State Farms Sugar Industrial Group Company Limited, Guangxi Sugarcane Industry R & D Center, Nanning, Guangxi, 530002, China) |
| Abstract: |
| [Objective] The aim of this paper is mainly to investigate the effects of glycerol on Pichia pastoris growth and constitutive expression of α-glucanase.[Methods] The initial concentration of glycerol in fermentation medium, glycerin concentration in fed-batch, ventilation and other effects on constitutive expression of α-glucanase were examined by means of single factor experiment.[Results] When the initial concentration of glycerol ranged from 50 g/L to 150 g/L, the growth and α-glucanase expression were not affected, while the growth and α-glucanase expression appeared to be markedly reduced when the glycerol increase to 200 g/L.Glycerol concentration between 0 to 5 g/L in fed-batch lead to the best growth and α-glucanase expression. When glycerin concentration became higher, the growth of Pichia pastoris and α-glucanase expression were restrained.Meanwhile, improving the ventilation was helpful to the expression of α-glucanase, and suitable fermentation time was 78 h.Under this condition, α-glucanase activity reached about 1763 U.[Conclusion] The process optimized the fermentation conditions for α-glucanase preparation with glycerol as carbon source, which greatly improved α-glucanase activity and laid a solid foundation for α-glucanase large-scale production. |
| Key words: Pichia pastoris α-glucanase glycerol fermentation conditions |
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