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  • 常国炜,梁达奉,张九花,曾练强,谭文兴,黎志德.发酵耦合酶解高效制备右旋糖酐工艺研究[J].广西科学,2014,21(6):619-623,633.    [点击复制]
  • CHANG Guo-wei,LIANG Da-feng,ZHANG Jiu-hua,ZENG Lian-qiang,TAN Wen-xing,LI Zhi-de.Study on Efficient Preparation for Dextran by Fermentation Coupled with Enzymatic Hydrolysis[J].Guangxi Sciences,2014,21(6):619-623,633.   [点击复制]
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发酵耦合酶解高效制备右旋糖酐工艺研究
常国炜, 梁达奉, 张九花, 曾练强, 谭文兴, 黎志德
0
(广州甘蔗糖业研究所, 广东省甘蔗改良与生物炼制重点实验室, 广东广州 510316)
摘要:
[目的]改进肠膜明串珠菌(Leuconostoc mesenteriodes 31208)发酵蔗糖制备右旋糖酐的工艺,实现灵活控制分子量及高效制备右旋糖酐的目标。[方法]采用单因素实验法研究蔗糖浓度对蔗糖转化的影响,确定蔗糖流加工艺的各个参数,以及右旋糖酐酶添加耦合蔗糖流加的工艺条件。[结果]通过调控右旋糖酐酶添加工艺,可灵活控制右旋糖酐的分子量,无需再经酸水解及乙醇分级沉淀就可以得到不同分子量的右旋糖酐,节省无水乙醇的用量;且确定的最佳蔗糖流加工艺参数:初始蔗糖浓度为130 g/L,流加速度为12 g·L-1·h-1,起始流加时间为16 h。与蔗糖单批发酵结果相比,该工艺可使蔗糖转化效率提高约75%,特定分子量(5000~7500 Da)右旋糖酐浓度可达108 g/L,相应提高2.3倍,收率稳定在32%左右。[结论]该方法具有发酵易调控、高效和成本低的优点,对大规模生产右旋糖酐具有重要的参考价值和应用价值。
关键词:  右旋糖酐  酶法  右旋糖酐酶  流加
DOI:
投稿时间:2014-10-10修订日期:2014-10-20
基金项目:国家863计划项目(2012AA021504)和八桂学者建设工程专项经费项目资助。
Study on Efficient Preparation for Dextran by Fermentation Coupled with Enzymatic Hydrolysis
CHANG Guo-wei, LIANG Da-feng, ZHANG Jiu-hua, ZENG Lian-qiang, TAN Wen-xing, LI Zhi-de
(Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, Guangdong, 510316, China)
Abstract:
[Objective] This paper aims to control dextran molecular flexibly and prepare dextran efficiently with Leuconostoc mesenteriodes 31208 by improving the preparation process.[Methods] The effect of sucrose concentration on sucrose conversion, the parameters of sucrose feeding, as well as the process conditions coupled enzymatic adding and sucrose feeding were studied by means of single factor experiment.[Results] The molecular weight of dextran was controlled flexibly by changing the enzymatic hydrolysis process, and the parameters of optimal sucrose feeding process were the initial sucrose concentration of 130 g/L with the flow acceleration of 12 g·L-1·h-1 at 16 h.Compared with the single batch fermentation, the conversion rate improved by 75% and specific-molecular dextran (5000~7500 Da)increased over time, and the yield of dextran was about 32%.[Conclusion] This method has the characteristics of simple fermentation control, high efficiency and low cost, which was important to the preparation of dextran for industrial production.
Key words:  dextran  enzymic method  dextranase  feeding

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