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孙雪萍,任义,徐艳,童万平,王长云.丛柳珊瑚Euplexaura rhipidalis中细胞毒活性二萜成分分析[J].广西科学,2013,20(4):269-271,275. [点击复制]
- SUN Xue-ping,REN Yi,XU Yan,TONG Wan-ping,WANG Chang-yun.Analysis of Cytotoxic Diterpenoid Composition from the Gorgonian Coral Euplexaura rhipidalis[J].Guangxi Sciences,2013,20(4):269-271,275. [点击复制]
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| 丛柳珊瑚Euplexaura rhipidalis中细胞毒活性二萜成分分析 |
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孙雪萍1,2, 任义3, 徐艳1, 童万平1, 王长云2
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| (1.广西海洋研究所海洋生物技术重点实验室, 广西北海 536000;2.中国海洋大学医药学院海洋药物教育部重点实验室, 山东青岛 266003;3.北海艺术设计职业学院环境艺术设计系, 广西北海 536000) |
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| 摘要: |
| 为了寻找结构新颖的海洋活性成分,对一种采自中国西沙海域丛柳珊瑚Euplexaura rhipidalis进行分离和纯化,并对获得的西松烷二萜化合物的抗肿瘤活性进行筛选,以探明该种丛柳珊瑚的活性组成成分。先利用正反相硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备HPLC等方法进行分离和纯化,再利用NMR、MS等波谱方法,并与文献对照确定了3个高氧化度的西松烷二萜类化合物,分别为corulloidolide A(1),(1R,3S,4S,7E,11E)-3,4-epoxycembra-7,11,15-triene(2),(-)-corulloidolide E(3);细胞毒活性模型评价结果发现,化合物3表现了较强的细胞毒活性,对5种癌细胞肝癌HepG2,Hep3B,乳腺癌阿霉素耐药株MCF-7/ADR,前列腺癌PC-3he,结肠癌HT-116的IC50值都低于10 μg/mL。表明西松烷二萜类化合物是该丛柳珊瑚E.rhipidalis的主要活性化合物类型之一。 |
| 关键词: 珊瑚 西松烷二萜 细胞毒活性 |
| DOI： |
| 投稿时间：2013-07-01修订日期：2013-08-26 |
| 基金项目:广西科学研究与技术开发计划项目(桂科攻11107011-8);广西科学院基本科研业务费项目(13YJ22HYS15);广西科学研究与技术开发计划(桂科攻11107011-6)项目资助。 |
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| Analysis of Cytotoxic Diterpenoid Composition from the Gorgonian Coral Euplexaura rhipidalis |
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SUN Xue-ping1,2, REN Yi3, XU Yan1, TONG Wan-ping1, WANG Chang-yun2
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| (1.Guangxi Institute of Oceanology, Guangxi Key Laboratory of Marine Biotechnology, Beihai, Guangxi, 536000, China;2.Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong, 266003, China;3.Environmental Art Design, Beihai Vocational Institute of Art & Design, Beihai, Guangxi, 536000, China) |
| Abstract: |
| The chemical constituents and their antitumor activities of Chinese gorgonian coral Euplexaura rhipidalis were preliminarily investigated in order to find more novel marine active ingredients.The ethanolic extract of E.rhipidalis was separated by repeated silica gel column chromatography, Sephadex LH-20 column chromatography and HPLC, by which three compounds were afforded.They were elucidated as three highly oxidized cembranoid diterpenoids:corulloidolide A (1), (1R, 3S, 4S, 7E, 11E)-3, 4-epoxycembra-7, 11, 15-triene (2) and (-)-corulloidolide E (3) by spectroscopic methods (NMR, MV) and by comparing with literatures.Compound 3 showed strong cytotoxicity to five strains of cancer cells including HepG2, Hep3B, MCF-7/ADR, PC-3he and HT-116 with the IC50 of less than 10 μg/mL.It was proposed that cembranoid diterpenoids should be one kind of the bioactive substances of gorgonian coral Euplexaura rhipidalis. |
| Key words: Euplexaura rhipidalis cembranoid diterpenoids cytotoxicity |
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