| 引用本文： |
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覃兰芳,赖茂祥,梁冰,屈信成,黄云峰,胡琦敏.高效液相色谱法测定广西壮药金花茶叶中的山奈素[J].广西科学,2012,19(4):345-347. [点击复制]
- QIN Lan-fang,LAI Mao-xiang,LIANG Bing,QU Xin-cheng,HUANG Yun-feng,HU Qi-ming.Determination of Kaempferol in Camellia petelotii by HPLC[J].Guangxi Sciences,2012,19(4):345-347. [点击复制]
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| 摘要: |
| 采用Ultimate(XB-C18)色谱柱,以甲醇-0.4%磷酸(55:45)为流动相,流速1.0 ml/min,检测波长360nm,柱温为室温,建立高效液相色谱法(HPLC)测定广西壮药金花茶叶中山奈素含量的方法。结果,山奈素在0.1~0.5μg范围内峰面积与其浓度呈良好线性关系(r=0.9998),平均回收率为96.36%,RSD=3.07%(n=6)。该方法准确,快速,重现性好,可以作为广西壮药材金花茶叶中山奈素含量的测定方法。 |
| 关键词: 含量测定 山奈素 金花茶 HPLC |
| DOI： |
| 投稿时间：2012-07-16修订日期：2012-08-13 |
| 基金项目:广西壮药质量标准研究项目(编号:MZY2010048)资助。 |
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| Determination of Kaempferol in Camellia petelotii by HPLC |
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QIN Lan-fang1, LAI Mao-xiang1,2, LIANG Bing1, QU Xin-cheng1, HUANG Yun-feng1, HU Qi-ming1
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| (1.Guangxi Institute of Chinese Medicine and Pharmaceutical Sciences, Nanning, Guangxi, 530022, China;2.Guangxi Key Laboratory of Traditional China Medicine Quality Standards, Nanning, Guangxi, 530022, China) |
| Abstract: |
| A HPLC method was established to determine kaempferol in Camellia petelotii (Merrll) Sealy.Ultimate(XB-C18)column was used as chromatographic column;The mobile phase was methanol-0.4%phosphoric acid solution (55:45);The flow rate was 1.0 ml·min-1 and the detector wavelength was set at 360 nm;The column temperature was maintained at room temperature.The calibration curve of kaempferol was in good linearity over the range of 0.1~0.5μg(r=0.9998).The average recovery was 96.36%, RSD=3.07%(n=6).This method is convenient, sensitive, reliable and suitable for determination of kaempferol in Camellia petelotii (Merrll) Sealy. |
| Key words: content determination kaempferol Camellia petelotii HPLC |
