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郑娟梅,李武钢,毛晓丽,王一兵,叶月华,黄庶识.傅里叶红外光谱法测定罗汉果中罗汉果总甙含量[J].广西科学,2011,18(4):355-358. [点击复制]
- ZHENG Juan-mei,LI Wu-gang,MAO Xiao-li,WANG Yi-bing,YE Yue-hua,HUANG Shu-shi.Determination of Mogrosides Contents in Siraitia grosvenorii with FTIR[J].Guangxi Sciences,2011,18(4):355-358. [点击复制]
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| 傅里叶红外光谱法测定罗汉果中罗汉果总甙含量 |
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郑娟梅1,2, 李武钢3,4, 毛晓丽5, 王一兵1, 叶月华2, 黄庶识1
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| (1.广西科学院, 广西南宁 530007;2.广西食品药品检验所, 广西南宁 530021;3.广西师范大学物理科学与技术学院, 广西桂林 541004;4.广西师范学院物理与电子工程学院, 广西南宁 530023;5.广西中医学院, 广西南宁 530001) |
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| 摘要: |
| 利用傅里叶变换红外光谱(FTIR)技术建立一种测定罗汉果中罗汉果总甙含量的新方法,通过最小二乘法拟合罗汉果总甙标准品红外光谱1059cm-1特征峰强度的标准曲线,得到回归方程,根据回归方程计算罗汉果样品甲醇超声波提取出物中的总甙含量。将计算结果分别进行精密度、重复性和稳定性检验,平行测定6次,罗汉果总甙的峰高相对标准偏差(RSD)分别为5.41%、5.47%和5.47%;FTIR法测定不同罗汉果样品的总甙含量与紫外分光光度计法的测定结果差异不显著(P>0.05)。本方法具有可行性与可靠性,能够满足实验室的准确度要求,可以作为罗汉果总甙定量测定的方法。 |
| 关键词: 罗汉果 总甙 红外光谱 定量分析 |
| DOI： |
| 投稿时间：2011-01-07 |
| 基金项目:广西科学研究与技术开发计划项目(桂科攻0992003A-20)资助 |
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| Determination of Mogrosides Contents in Siraitia grosvenorii with FTIR |
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ZHENG Juan-mei1,2, LI Wu-gang3,4, MAO Xiao-li5, WANG Yi-bing1, YE Yue-hua2, HUANG Shu-shi1
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| (1.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Guangxi Institute for Food and Drug Control, Nanning, Guangxi, 530021, China;3.College of Physics Sciences and Technology, Guangxi Normal University, Guilin, Guangxi, 541004, China;4.College of Physics and Electronics Engineering, Guangxi Teachers Education University, Nanning, Guangxi, 530023, China;5.Guangxi Traditional Chinese Medical University, Nanning, Guangxi, 530001, China) |
| Abstract: |
| Fourier transform infrared spectroscopy (FT-IR) was utilized as a new approach to investigate the mogrosides contents of Siraitia grosvenorii.A serial intensity value of 1059 cm-1 peak, which was intended to be the characteristic peak of mogrosides within its IR fingerprint spectra, was obtained from the IR spectra of different contents of mogrosides standards and used to draw standard curve as a function of mogrosides contents, in which the curve was fitted with least square fitting (LSF) for establishing linear regression equation.The concentration of mogrosides extracted from fructus momordicae sample with methanol combining ultrasonic method was determined by using the regression equation.The relative standard deviation (RSD) was calculated from 6 replicate determinations, of which the precision, repeatability and stability were 5.41%, 5.47% and 5.47%, respectively.The mogrosides contents measured from different Siraitia grosvenorii samples by FT-IR were not significant differences compared with those determined by UV spectrophotometry (P> 0.05), suggesting that FT-IR is a feasible method for quantificational detection of mogrosides content in Siraitia grosvenorii. |
| Key words: Siraitia grosvenorii mogrosides infrared spectra quantitative analysis |
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