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黄靖华,孙靓,吴军华,孙菲菲,李检秀,黄艳燕,郭铃,黄日波.甘蔗糖蜜发酵生产L-乳酸工艺条件研究[J].广西科学,2011,18(3):273-277. [点击复制]
- HUANG Jing-hua,SUN Liang,WU Jun-hua,SUN Fei-fei,LI Jian-xiu,HUANG Yan-yan,GUO Ling,HUANG Ri-bo.Fermentation Condition Studies on the Production of L-lactic Acid from Sugarcane Molasses[J].Guangxi Sciences,2011,18(3):273-277. [点击复制]
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| 甘蔗糖蜜发酵生产L-乳酸工艺条件研究 |
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黄靖华1, 孙靓1,2, 吴军华3, 孙菲菲2, 李检秀2, 黄艳燕2, 郭铃2, 黄日波1,2
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| (1.广西大学生命科学与技术学院, 广西南宁 530004;2.广西科学院非粮生物质酶解国家重点实验室, 国家非粮生物质能源工程技术研究中心, 广西生物炼制重点实验室, 广西南宁 530007;3.广西科学院, 广西南宁 530007) |
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| 摘要: |
| 以甘蔗糖蜜作为原料,利用经诱变筛选得到的鼠李糖乳杆菌SCT-10-10-60发酵生产L-乳酸。通过设置单因素实验,研究不同初始糖蜜浓度(50%、40%、30%、20%)、不同发酵温度(30℃、37℃、42℃、47℃)、不同pH调节剂碳酸钙加入量(4%、6%、8%、10%)和氮源替代(尿素替代酵母粉)对L-乳酸产量的影响。结果表明,在初始糖蜜浓度20%(m/V)、温度37℃、碳酸钙浓度4%(m/V)、鼠李糖乳杆菌SCT-10-10-60接种量6%(V/V)的发酵条件下,发酵40h L-乳酸的产量最高可以达到106g/L。尿素不能作为菌株生长所需要的氮源。 |
| 关键词: L-乳酸 甘蔗糖蜜 鼠李糖乳杆菌 |
| DOI： |
| 投稿时间：2011-01-07 |
| 基金项目:国家科技支撑计划项目(2007BAD75B06);广西科学基金(桂科攻0782003-4);广西科学院基本科研业务项目(10YJ25SW11)资助 |
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| Fermentation Condition Studies on the Production of L-lactic Acid from Sugarcane Molasses |
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HUANG Jing-hua1, SUN Liang1,2, WU Jun-hua3, SUN Fei-fei2, LI Jian-xiu2, HUANG Yan-yan2, GUO Ling2, HUANG Ri-bo1,2
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| (1.College of Life Science and Techology, Guangxi University, Nanning, Guangxi, 530004, China;2.State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Science;3.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| The fermentation conditions of producing L-lactic acid with sugarcane molasses as feedstock were investigated using mutated Lactobacillus rhamnosus SCT-10-10-60.By the single factor experiments, optimal fermentation conditions were investigated.Experimental results showed that under conditions of 20% molasses concentration (m/V), 37℃ fermentation temperature, 4% (m/V) calcium carbonate addition, 6% (V/V) inoculum concentration, and other fermentation conditions, the maximum production of L-lactic acid can reach to 106g/L in 40 hours.Experimental results also show that urea can not substitute the yeast exacts as nitrogen sources for the growth of bacteria.In addition, fermentation broth can maintain liquid but not coagulate in the late state, which is conducive to the separation and purification of final products.Therefore, L-lactic acid production from cheap sugarcane molasses is prospective. |
| Key words: L-lactic acid sugarcane molasses Lactobacillus rhamnosus |
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