| 摘要: |
| 采用响应面法对醋酸钙不动杆菌(Acinetobacter calcoaceticus)菌株23的发酵产脂肪酶培养基进行优化实验。实验首先考察该菌株产酶所需的最适碳源和氮源,然后用Plackett-Burman法设计实验确定影响产酶的主要影响因素,用最陡爬坡实验逼近最大响应区域,最后通过Box-Behnken方法进行二次回归分析得到最佳的产酶条件为:营养肉汤18.4%、蛋白胨9.18%、吐温800.52%、K2HPO4 0.2%,橄榄油1%,MgSO4 0.05%,CaCl2 0.05%,FeSO4 0.1%。在该优化条件下,发酵液的脂肪酶活力可以达到15.2U/ml,比优化前提高3倍。 |
| 关键词: 脂肪酶 醋酸钙不动杆菌 Plackett-Burman设计 响应面 优化 |
| DOI： |
| 投稿时间：2008-01-27 |
| 基金项目:广西科学院创新基金项目(No.桂科院研0701)资助 |
|
| The Lipase Production Optimization of Acinetobacter calcoaceticus 23 Using Response Surface Methodology |
|
ZHANG Bo
|
| (Guangxi Academy of Science, Nanning, Guangxi, 530007, China) |
| Abstract: |
| Response surface methodology (RSM)was employed to optimize culture medium for lipase production of Acinetobacter calcoaceticus 23. Firstly, the best carbon and nitrogen sources was investigated. Then a Plackett-Burman design was used to investigate the effects of different factor in the ferment medium, the 3 significtant is:broth, peptone, tween 80, and then steepest ascent procedures were employed to define optimal response region for these three factors. Finally, Box-Behnken was employed to design 17 experiments. The results of experiments was analyzed with Design-expert soft. The optimal lipase production medium is:18.4% nutrient broth, 9.18% tryptone, 0.52%, tween 80 0.2%, K2HPO4, 1%, olive oil 1%, MgSO4 0.05%, CaCl2 0.05%, FeSO4 0.1%, the yield of enzyme activity achieved 15.2 U/ml, the yield is increased by 3 times. |
| Key words: lipase Acinetobacter calcoaceticus Plackett-Burman response surface methodology optimization |
