| 引用本文： |
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苏龙,韦宇拓,黄日波.广西象州温泉嗜热菌的分离与鉴定[J].广西科学,2006,13(4):327-329,333. [点击复制]
- SU Long,WEI Yu-tuo,HUANG Ri-bo.Separation and Identification of Thermophilic Bacterium Strain from Xiangzhou Hot Springs of Guangxi[J].Guangxi Sciences,2006,13(4):327-329,333. [点击复制]
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| 摘要: |
| 采集广西象州温泉水样,用常规的稀释涂布法分离和纯化嗜热菌,并选择其中1株编号为XZ2K2的菌株进行形态观察、生理生化鉴定和16S rDNA序列分析。结果从温泉水中分离得到9个菌株,XZ2K2菌株的革兰氏染色阳性,无芽孢,细胞呈杆状,最适生长温度为70~75℃,最适的生长pH值为7.0~7.5.初步鉴定该菌株为布氏栖热菌(Thermus brockianus)。 |
| 关键词: 嗜热菌 分离 鉴定 温泉 |
| DOI： |
| 投稿时间：2006-04-28修订日期：2006-07-10 |
| 基金项目: |
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| Separation and Identification of Thermophilic Bacterium Strain from Xiangzhou Hot Springs of Guangxi |
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SU Long1, WEI Yu-tuo2, HUANG Ri-bo2
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| (1.Department of Chemistry and Biology, Yulin Teachers College, Yulin, Guangxi, 537000, China;2.College of Life Science and Biotechnology, Guangxi University, Nanning, Guangxi, 530005, China) |
| Abstract: |
| In order to separate and identify the thermophilic lecteria in hot springs of Xiangzhou,Guangxi,the general method of dilution spread was used.One strain was selected and labelled as XZ2K2.Morphological observation,physiology-biochemistry tests and 16SrDNA sequence analysis have been conducted.Nine strains were separated from the hot springs.XZ2K2 is bacillus,gram-positive,non-sporulation.Its optimum temperature for growth was between 70℃ and 75℃,optimum pH for growth was between 7.0 and 7.5.XZ2K2 was identified as Thermus brockianus. |
| Key words: themophilic bacteria separation identification hot springs |
