| 引用本文： |
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陈全斌,程忠泉,杨建香,义祥辉.罗汉果种仁油中角鲨烯的高效液相色谱分析[J].广西科学,2006,13(2):118-120. [点击复制]
- CHEN Quan-bin,CHENG Zhong-quan,YANG Jian-xiang,YI Xiang-hui.The HPLC Analysis of the Squalene in Siraitia grosvenorii Seed Oil[J].Guangxi Sciences,2006,13(2):118-120. [点击复制]
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| 摘要: |
| 采用高效液相色谱法分析测试罗汉果(Siraitia grosvenorii)种仁油中的角鲨烯,确立色谱条件为:色谱柱:Shodex Asahipak C18,流动相为甲醇/乙腈=60/40、检测波长为203nm、流速为2ml/min、柱温为40℃。在此条件下各峰得到良好的分离,标准曲线具有良好的线形关系,r=0.9973;精密度RSD=3.45%(n=5);回收率达98.48%。 |
| 关键词: 罗汉果 种仁油 角鲨烯 高效液相色谱 |
| DOI： |
| 投稿时间：2006-01-12修订日期：2006-03-08 |
| 基金项目:广西教育厅科研项目(桂教科研[2004]20号);广西师范大学重点项目(师政2005,第1号)联合资助 |
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| The HPLC Analysis of the Squalene in Siraitia grosvenorii Seed Oil |
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CHEN Quan-bin1, CHENG Zhong-quan2, YANG Jian-xiang2, YI Xiang-hui2
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| (1.Department of Resources and Environment, Guangxi Normal University, Guilin, Guangxi, 541004, China;2.Guilin Normal College, Guilin, Guangxi, 541001, China) |
| Abstract: |
| The analysis and preparation condition of squalene in Siraitia grosvenorii seed oil by HPLC are estabilished in this thesis, the following HPLC conditions are the best:column:Shodex Asahipak C18 column;mobile phase, V (acetonitrile):V (methanol)=60:40;detection wavelength, 203nm;flow rate, 2ml/min;tmperature, 40℃.Every components are separated on this condition, and the standard curve has good linear relation, r=0.9973;exactitude degree, RSD=3.45% (n=5);erecycle rate is 98.48%. |
| Key words: Siraitia grosvenorii seed oil squalene HPLC |
