| 摘要: |
| 为了研究静电对紫花苜蓿(Medicago sativa Leguminosae)抗旱性的影响,寻找出提高紫花苜蓿幼苗抗旱性的最佳电场条件。在静电场不同电场强度下处置紫花苜蓿种子10min后,室温下培养9d,再测定幼苗的丙二醛(MDA)的含量和过氧化物酶(POD)、超氧化物酶(SOD)的活性。实验所用电极电压波形为50Hz半波整流,电场强度共11个,其中1个未经电场处理作为对照组。结果表明,在11个电场处理的材料中,有6个MDA水平低于对照,1个与对照相同,其余4个高于对照,变化幅度为22.8%~34.8%。SOD酶和POD酶的活性普遍降低,降低幅度分别为7.7%~57.7%和0.5%~59.4%。静电场处理可以提高紫花苜蓿幼苗的抗旱性。 |
| 关键词: 苜蓿种子 酶活性 抗旱性 静电场 |
| DOI： |
| 投稿时间：2004-04-05修订日期：2004-08-23 |
| 基金项目: |
|
| Study on Electrostatic Field on Isoenzyme Activity of Medicago sativa Leguminosae in Its Seeding Stage |
|
Tao Yuhua
|
| (Guangxi Vocational Ecology & Technical College, Liuzhou, Guangxi, 545004, China) |
| Abstract: |
| This paper studies the effects of electrostatic field on drought resistance of Medicago sativa Leguminosae and find out the best way of electrostatic field to improve drought resistance of Medicago sativa Leguminseae.Alfala seeds are treated by electrostatic field with 11 different strength for about 10 minutes.One is the contrast.After culturing for 9 days in the room temperature, the activity of their POD and SOD as well as the contents of MAD(malon-di-aldehyde) can be tested.The results show that in the normal moisture content and room temperature,the electrostatic field treatment is positively related with MDA.The MDA which comes from 6 alalfa having been treated is lower than that non-treated.The changes of MAD is between 22.8% and 34.8%.However,the activity of POD and SOD decreased compared with the contrast after electrostatic treatment.The changes of SOD is between 7.7% and 57.7%.The changes of POD is between 0.5% and 59.4%.Electrostatic treatment can improve the drought resistance of Medicago sativa Leguminosae in its seeding stage. |
| Key words: alfala seeds isoenzyme activity drought resistance electrostatic field |
