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  • 陈全斌,杨建香,程忠泉,义祥辉.RP-HPLC法测定罗汉果叶中总黄酮含量[J].广西科学,2005,12(1):43-45.    [点击复制]
  • Chen Quanbin,Yang Jianxiang,Cheng Zhongquan,Yi Xianghui.The Determination of Total Flavonoids in Momordica grosvenori Leaf by RP-HPLC[J].Guangxi Sciences,2005,12(1):43-45.   [点击复制]
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RP-HPLC法测定罗汉果叶中总黄酮含量
陈全斌, 杨建香, 程忠泉, 义祥辉
0
(广西师范大学资源与环境科学系, 广西桂林 541004)
摘要:
用反相高效液相色谱法(HPLC)测定罗汉果(Momordica grosvenori)叶中总黄酮的含量,用保留时间定性,用外标法定量。结果表明,罗汉果叶含有黄酮甙元槲皮素和山奈酚,槲皮素线性回归方程为y=179033.73x+6958.30,r=0.9951;山奈酚线性回归方程为y=176444.32x+10827.99,r=0.9944。罗汉果叶中总黄酮含量为1.618%。
关键词:  罗汉果叶  总黄酮  HPLC  定量
DOI:
投稿时间:2004-05-28
基金项目:广西科学技术厅研究与开发计划(桂科能0443001-12)资助项目。本课题中罗汉果叶黄酮开发与应用,已申请国家专利(200310110827.X)。
The Determination of Total Flavonoids in Momordica grosvenori Leaf by RP-HPLC
Chen Quanbin, Yang Jianxiang, Cheng Zhongquan, Yi Xianghui
(Dept. of Natural Reso. & Envi. Sci., Guangxi Normal Univ., Guilin, Guangxi, 541004, China)
Abstract:
Quercetin and kaempferol are found from Momordica grosvenori leaves by their Retain-time in RP-HPLC.The contents of total flavonoids is measured by external standard method,and the result is 1.618%.
Key words:  Momordica grosvenori leaf  total flavonoid  HPLC  quantitative

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