| 引用本文： |
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张桂英,廖咏梅,张君成.甘蔗黑穗病菌拮抗性芽孢杆菌的抗菌作用与伊枯草菌素A的产生有关[J].广西科学,2004,11(3):269-272. [点击复制]
- Zhang Guiying,Liao Yongmei,Zhang Juncheng.The Antifungal Mechanism of Antagonistic Bacillus subtilus to Ustilago scitaminea is Related to Iturin A Producing[J].Guangxi Sciences,2004,11(3):269-272. [点击复制]
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| 摘要: |
| 选择对甘蔗黑穗病菌有拮抗作用的5个革兰氏阳性芽孢杆菌拮抗菌株和3个革兰氏阴性拮抗细菌菌株,用PCR技术,扩增抗霉菌枯草杆菌素操纵元中mycB基因。结果是,5个革兰氏阳性芽孢杆菌拮抗菌株均扩增出一条大小为2.0kb左右的特异带,革兰氏阳性芽孢杆菌基因组的PCR产物和伊枯草菌素A合成酶操纵元中的ItuB基因的同源性为97%~98%,说明其抗菌作用机制和Iturin类群脂肽抗生素的产生有关。3个革兰氏阴性拮抗细菌中,没有扩增到基因,其抗菌作用机制有待进一步研究。 |
| 关键词: 甘蔗黑穗病菌 拮抗细菌 伊枯草菌素A |
| DOI： |
| 投稿时间：2004-03-31修订日期：2004-06-22 |
| 基金项目:广西自然科学基金资助项目(桂科自9912015)。 |
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| The Antifungal Mechanism of Antagonistic Bacillus subtilus to Ustilago scitaminea is Related to Iturin A Producing |
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Zhang Guiying, Liao Yongmei, Zhang Juncheng
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| (Agric. College, Guangxi Univ., 100 Daxuelu, Nanning, Guangxi, 530004, China) |
| Abstract: |
| 5 gram-positive Bacillus strains and 3 gram-negative bacteria strains which are antagonistic to Ustilago scitaminea were selected to be amplified the mycB gene of iturin A operon by PCR.A band of about 2.0 kb appeared among all strains of 5 gram-positive Bacillus.The PCR product is 97%~98% homologous to ItuB gene of iturin A operon.This result shows that the antifungal mechanism of antagonistic Bacillus is related to Iturin group lipopeptin producing.There are no any PCR products appearing among 3 gram-negative bacteria.More research on their antifungal mechanism need to be done in the future. |
| Key words: Ustilago scitaminea antagonistic bacteria Iturin A |
