| 引用本文： |
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蒋丽娟,孙友平,李培旺.培养因子对绿玉树离体微型快繁的影响[J].广西科学,2003,10(3):232-237. [点击复制]
- Jiang Lijuan,Sun Youping,Li Peiwang.Effects of Culture Factors on Micro-propagation of Euphorbia tirucalli[J].Guangxi Sciences,2003,10(3):232-237. [点击复制]
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| 摘要: |
| 以绿玉树的腋芽茎段和顶芽为外植体,研究不同培养基浓度、不同激素配比、外植体的放置方式以及化学物质PEG、PP333、AgNO3等因子对绿玉树离体培养丛生芽产生的影响,以此筛选出绿玉树离体快繁最适培养基及培养途径。结果表明:诱导腋芽、顶芽萌发较理想的培养基为:3/4MS+ZT0.5~1mg/L+NAA0.01mg/L和1/2MS+BA0.5mg/L+NAA0.01mg/L,适宜丛生芽增殖的培养基为:MS+ZT0.5~2mg/L+NAA0.05mg/L+GA30.01mg/L,芽的增殖系数为4.5。 |
| 关键词: 绿玉树 微型快繁 培养基 增殖系数 |
| DOI： |
| 投稿时间：2003-05-27 |
| 基金项目:国家林业局"948"项目和"863"项目《能源植物及液体燃料利用新技术研究示范》资助。 |
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| Effects of Culture Factors on Micro-propagation of Euphorbia tirucalli |
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Jiang Lijuan, Sun Youping, Li Peiwang
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| (Hunan Academy of Forestry, Changsha, Hunan, 410004, China) |
| Abstract: |
| The effects of contents of mediums,phytohormone,position of explant on the mediums and the chemical such as PEG,PP 333,AgNO3 on the regeneration of cluster buds were studied by using the stem fragment with auxiliary buds and shoot tip as explants.The results show that mediums of 3/4MS+ZT0.5~1 mg/L+NAA0.01mg/L and 1/2MS+BA0.5mg/L+NAA0.01mg/L,were suitble for the geminating of shoot tips and auxiliary buds.Mediums of MS+ZT0.5~2mg/L+NAA0.05mg/L+GA3 0.01mg/L was optimum for reproducing cluster buds,and the coefficient of plantlet regeneration was up to 4.5. |
| Key words: Euphorbia tirucalli micro-propagation mediums regeneration coefficient |
