| 引用本文： |
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周传明,黄寿先,熊英,黄文.火龙果茎段离体培养快速繁殖试验[J].广西科学,2002,9(1):78-80. [点击复制]
- Zhou Chuanming,Huang Shouxian,Xiong Ying,Huang Wen.Rapid Propagation of Hylocereus undatus cv.vietnam by In Vitro Stem Sections[J].Guangxi Sciences,2002,9(1):78-80. [点击复制]
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| 摘要: |
| 选取火龙果当年春季抽出的未老熟茎段作外植体,以MS为基本培养基,诱导腋芽生长及继代培养时附加6-BA和NAA,6-BA浓度为1.0~12.0mg/L,NAA浓度为0.2~0.8mg/L;诱导生根时附加IBA,IBA浓度为0.5mg/L、1.0mg/L、1.5mg/L。试验结果表明:以MS基本培养基附加6-BA2.0mg/L+NAA0.2mg/L有利于诱导腋芽生长;附加6-BA12.0mg/L+NAA0.1mg/L有利诱导增殖和继代;附加IBA1.5mg/L诱导生根最好,生根培养7d后开始生根,培养20d生根率达96.7%。将火龙果生根苗移栽到菜园土和细砂基质,成活率均达100%,以细砂为基质的移栽苗木生长较好,说明火龙果的组培苗移栽需要有良好的透水,透气条件。 |
| 关键词: 火龙果 茎段 组织培养 繁殖 |
| DOI： |
| 投稿时间：2001-09-04修订日期：2001-10-25 |
| 基金项目: |
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| Rapid Propagation of Hylocereus undatus cv.vietnam by In Vitro Stem Sections |
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Zhou Chuanming, Huang Shouxian, Xiong Ying, Huang Wen
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| (Forestry College, Guangxi Univ., 16 Yongwulu, Nanning, Guangxi, 530001, China) |
| Abstract: |
| Tender stems of Hylocereus undatus cv. vietnam are taken as explants MS medium(MS) is used and added with 6-BA of 1.0 to 12.0 mg/L and NAA of 0.2 to 0.8 mg/L to induce axillary buds'growth and subculture, and supplemented with IBA at the concentrations 0.5 mg/L,1.0 mg/L and 1.5 mg/L to induce rooting. The experiment shows that MS+6-BA 2.0 mg/L+NAA 0.2 mg/L is suitable for growth of axillary buds, and MS+6-BA 12.0 mg/L+NAA 0.1 mg/L suitable for subculture and, MS+IBA1.5 mg/L suitable for induction of rooting. The shoots root on 7th day after transplanted, and reach 96.7% of rooting rate on 20th day after transplanted. All plantlets are survival when they are transplanted into the soil of vegetable garden and sand medium,but the plantlets grow better in the sand medium than in the soil. |
| Key words: Hylocereus undatus cv.vietnam stem sections tissue culture propagation |
