| 引用本文： |
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蒙健宗,李晓明,王青艳,卢福燊,周志强,李庆业,李丛,韦函忠,黄日波.高密度培养基因工程大肠杆菌生产α-乙酰乳酸脱羧酶[J].广西科学,2001,8(4):284-286,290. [点击复制]
- Mong Jianzong,Li Xiaoming,Wang Qingyan,Lu Fushen,Zhou Zhiqiang,Li Qingyie,Li Cong,Wei Hanzhong,Huang Ribo.Produce of α-Acetolactate Decarboxylase in Engineering Escherichia coli by High Density Culture[J].Guangxi Sciences,2001,8(4):284-286,290. [点击复制]
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| 摘要: |
| 在3000L发酵罐中,利用分批补料培养技术高密度培养含分泌型重组质粒p ETGAU-10的大肠杆菌TG1,生产α-乙酰乳酸脱羧酶。通过控制发酵条件,发酵液的细胞密度(OD600)可达30.5,且胞外α-乙酰乳酸脱羧酶活力最高达890U·ml-1,达到较为理想的工业化生产的要求。 |
| 关键词: α-乙酰乳酸脱羧酶 分批补料 高密度培养 基因工程菌 胞外酶 |
| DOI： |
| 投稿时间：2001-08-29修订日期：2001-10-11 |
| 基金项目:中国高技术研究发展计划(863计划)资助项目(101-06-07-01)。 |
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| Produce of α-Acetolactate Decarboxylase in Engineering Escherichia coli by High Density Culture |
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Mong Jianzong, Li Xiaoming, Wang Qingyan, Lu Fushen, Zhou Zhiqiang, Li Qingyie, Li Cong, Wei Hanzhong, Huang Ribo
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| (Institute of Ferment and Enzyme Engineering, Guangxi University, 13 Xiulinglu, Nanning, Guangxi, 530005, China) |
| Abstract: |
| Escherichia coli TG1,which contained secreted recombinant plasmid pETGAU-10,was cultured to a high density to produce α-ALDC by the fed-batch culture in 3000 L fermentor. In control of the fermentative conditions,the cell density can reach as high as 30.5 of OD600 and the highest extracellular activity of α-ALDC reached 890 U·ml-1,which meet the demand for the ideal industrial production. |
| Key words: ALDC fed batch culture high density culture engineering bacteria extracellular enzyme |
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