| 引用本文： |
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杨海波,樊晓晖,何先保,黄企光,黎肇炎,Franc Gubensek.桂北五步蛇纤溶酶金属蛋白酶原基因的初步研究[J].广西科学,2000,7(4):307-308,318. [点击复制]
- Yang Haibo,Fan Xiaohui,He Xianbao,Huang Qiguang,Li Zhaoyan,Franc Gubensek.Primary Study of cDNA Encoding Fibrinolysin Metalloproteinase from the Venom of Agkistrodon acutus[J].Guangxi Sciences,2000,7(4):307-308,318. [点击复制]
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| 摘要: |
| 从桂北五步蛇毒腺中抽提蛇毒总RNA,采用一步法(RT-PCR和PCR在同一试管内进行)扩增纤溶酶金属蛋白酶原基因,并进行电泳检测,可见3条特异的DNA条带,分别约为1.5 Kb、1.3Kb和800bp,利用平端连接的方法将其中的800bp PCR产物克隆至pGEM-T Easy载体,挑选白色菌落,用酶切和PCR法对其进行鉴定。 |
| 关键词: 五步蛇 纤溶酶金属蛋白酶原 PCR 基因克隆 |
| DOI： |
| 投稿时间：2000-05-18修订日期：2000-07-17 |
| 基金项目:广西青年科学基金会资助项目(桂青自9912018)。 |
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| Primary Study of cDNA Encoding Fibrinolysin Metalloproteinase from the Venom of Agkistrodon acutus |
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Yang Haibo, Fan Xiaohui, He Xianbao, Huang Qiguang, Li Zhaoyan, Franc Gubensek
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| (Guangxi Medical University, 6 Binhulu, Nanning, Guangxi, 530021, China) |
| Abstract: |
| One step method was used to extract total RNA from the venom of Agkistrodon acutus which was found in the northern mountain area of Guangxi province. The cDNAs encoding fibrinolysin metalloproteinase were amplified by one step method (RT-PCR and PCR reactions occurred in the same test tube). The PCR product is about 1.5 Kb,1.3 Kb and 800bp.The 800 bp PCR product was cloned into the pGEM-T Easy vector. Plasmid DNA was obtained from positive clones, identified by means of digestion with EcoR I and PCR reaction. |
| Key words: Agkistrodon acutus fibrinolysin metalloproteinase PCR gene clone |
