| 引用本文： |
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刘华钢,陈燕军,张建云,张永贵.反相高效液相色谱法测定天和骨通膏中阿魏酸的含量[J].广西科学,2000,7(4):304-306. [点击复制]
- Liu Huagang,Chen Yanjun,Zhang Jianyun,Zhang Yonggui.Determination of Ferulic Acid Content of Tianhe Gutong by RP-HPLC[J].Guangxi Sciences,2000,7(4):304-306. [点击复制]
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| 摘要: |
| 为建立反相高效液相色谱法测定中药橡皮膏天和骨通中阿魏酸含量的测定方法,采用天和骨通的甲醇-甲酸(95:5)提取液,通过Shim-pack CLC-ODS柱,以甲醇-水-冰醋酸(45:55:1)为流动相,在UV-322 nm波长处检测阿魏酸的含量。测定结果显示,阿魏酸含量在2.44μg/ml~12.20μg/ml的范围内有良好的线性关系,r=0.9999。平均加样回收率为101.4%,RSD为1.54%。试验表明本方法快速、简便、准确,可用于天和骨通中阿魏酸的含量测定。 |
| 关键词: 中药橡皮膏 天和骨通 阿魏酸 反相高效液相色谱法 含量测定 |
| DOI： |
| 投稿时间：2000-04-10修订日期：2000-08-02 |
| 基金项目: |
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| Determination of Ferulic Acid Content of Tianhe Gutong by RP-HPLC |
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Liu Huagang, Chen Yanjun, Zhang Jianyun, Zhang Yonggui
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| (Guangxi Medical University, 6 Binhulu, Nanning, Guangxi, 530021, China) |
| Abstract: |
| To establish a technique for determination of ferulic acid content of Tianhe Gutong,Chinese medicine adhesive plaster,by the reversed-phase HPLC, the extraction of methanol-formic acid (95:5) of Tianhe Gutong was used to pass through the Shim-pack CLC-ODS volume.A mixture of methanol-water-glacialaceticacid (45:55:1) was used as mobile phase. The ferulic acid content was determined at UV 322 nm. The standard curve was linear in the range of 2.44 μg/ml to 12.20 μg/ml, r=0.9999.The average recovery was 101.4% and RSD was 1.54%. This method is rapid, simple and accurate, and satisfys the determination of ferulic acid content of Tianhe Gutong. |
| Key words: Chinese medicine adhesive plaster Tianhe Gutong ferulic acid RP-HPLC content determination |
