| 引用本文： |
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黄华艺,钟鸣,农朝赞,赵世元,孟刚.脂质过氧化大鼠脑组织超微结构改变及杧果甙对脑组织的保护作用[J].广西科学,2000,7(2):128-130. [点击复制]
- Huang Huayi,Zhong Ming,Nong Chaozan,Zhao Shiyuan,Meng Gang.The Ultramicro-structure Change of Lipid Superoxided Rat Brain Tissue and Protect of Mangiferin on the Tissues[J].Guangxi Sciences,2000,7(2):128-130. [点击复制]
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| 摘要: |
| 为了探讨过氧化脂质对大鼠脑组织超微结构的影响以及杧果甙对脑组织的保护作用。用四氧嘧啶建立脂质过氧化动物模型,大鼠分:(A)空白对照组:动物只给5%淀粉水溶液10ml/d灌胃21d;(B)杧果甙高剂量组:动物每天给予杧果甙30mg/kg灌胃21d;(C)杧果甙低剂量组:动物每天给予杧果甙15 mg/kg灌胃21d;(D)阳性对照组:动物每天给予维生素E75 mg/kg灌胃21d;(E)模型组:动物给予生理盐水10ml/d灌胃21d。除A组外,均于实验后第16天起每天腹腔注射四氧嘧啶75 mg/kg,第21天将各组动物进行灌注固定,观察尾状核神经元超微结构改变。结果A组动物脑组织神经元细胞器形态正常,结构清楚,突触密度数(Nv)为(1.233±0.102)个/微米3;B组动物脑组织神经元各项指标与A组差别不大,Nv为(1.342±0.098)个/微米3;C组动物脑组织神经元线粒体嵴减少,嵴间隙增宽,突触密度数减少,Nv为(0.8376±0.075)个/微米3;D组动物脑组织神经元各项指标与A组差别不大,但线粒体嵴增宽,Nv为(1.221±0.092)个/微米3;E组动物脑组织神经元线粒体嵴减少,嵴间隙增宽,突触密度数明显减少,Nv为(0.8262±0.058)个/微米3。说明杧果甙具有清除过氧化脂质作用,减轻过氧化脂质对神经元的损伤,从而保护神经元的正常功能。 |
| 关键词: 杧果甙 过氧化脂质 细胞超微结构 |
| DOI： |
| 投稿时间：1999-09-29 |
| 基金项目:广西壮族自治区科学技术厅青年科学基金资助(桂科青9736009)。 |
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| The Ultramicro-structure Change of Lipid Superoxided Rat Brain Tissue and Protect of Mangiferin on the Tissues |
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Huang Huayi, Zhong Ming, Nong Chaozan, Zhao Shiyuan, Meng Gang
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| (Guangxi Nationalities Hospital, 34 East Mingxiulu, Nanning, Guangxi, 530001, China) |
| Abstract: |
| To investigate the ultramicro-structure change of lipid superoxided rat brain tissue and protect of mangiferin on the tissues, we established a lipid superoxided model for rat using chemical substance-alloxan for our study.Rats were divided into 5 groups:Group A:the animals were gastrogavaged 10 ml/day of 5% starch solution for 21 days.Group B:30 mg/kg/day of mangiferin for 21 days.Group C:15 mg/kg/day of mangiferin for 21 days.Group D:75 mg/kg/day of Vit E for 21 days.Group E:10 ml/day of saline for 21 days,and Groups B to E were also intraperitoneal injected 75 mg/kg/day of alloxan from day 16 to day 21 The animals were then executed for perfuse fixation,and ultramicro-structure change in caudate nucleus of brain tissue were observed.The forms of organelles in neurons of Group A were normal, Nv:(1.233±0.102)/μm3.,while it showed that Group B's were roughly normal,Nv:(1.342±0.098)/μm3.The mitocondrial cristae in neurons of Group C were reduced,the width between crista increased.Nv reduced(0.8376±0.075).The organelles in neurons of Group D were roughly normal,but the width between crista increased,Nv:(1.221±0.092). Organelles in neurons of Group E were abnormal,cristae reduced,the width between crista increased significantly,and the Nv also reduced significantly(0.8262±0.058).Mangiferin can scavenge lipids superoxides from brain tissue,and protect the normal function of neuron. |
| Key words: mangiferin superoxide cell ultramicro-structure |
