| 引用本文： |
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涂楚桥,孙红莲,曾建强,梁宏.Zn(Ⅱ)与人血清白蛋白或牛血清白蛋白的结合平衡研究[J].广西科学,1999,6(4):278-280. [点击复制]
- Tu Chuqiao,Sun Honglian,Zeng Jianqiang,Liang Hong.Binding Equilibrium of Zn(Ⅱ) to Human Serum Albumin and Bovine Serum Albumin[J].Guangxi Sciences,1999,6(4):278-280. [点击复制]
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| 摘要: |
| 用平衡透析法研究了生理条件(pH值7.43)下Zn(Ⅱ)与人血清白蛋白(HSA)或牛血清白蛋白(BSA)的相互作用。结果表明,HSA对Zn(Ⅱ)有1.1个强结合部位和9.0个弱结合部位,结合常数分别为1.04×105及1.67×103;BSA对Zn(Ⅱ)有1.2个强结合部位和约9.7个弱结合部位,结合常数分别为8.2×104及1.9×103。Zn(Ⅱ)-HSA和Zn(Ⅱ)-BSA的Hill系数分别为0.87、0.84,表明Zn(Ⅱ)与HSA或BSA的结合均只产生较小的负协同效应。 |
| 关键词: 锌(Ⅱ) 血清白蛋白 平衡透析 协同效应 |
| DOI： |
| 投稿时间：1999-02-01 |
| 基金项目:国家自然科学基金、广西十百千人才基金和广西高校自然科学基金资助项目。 |
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| Binding Equilibrium of Zn(Ⅱ) to Human Serum Albumin and Bovine Serum Albumin |
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Tu Chuqiao, Sun Honglian, Zeng Jianqiang, Liang Hong
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| (Dept. of Chemistry and Chemical Engineering, Guangxi Normal University, 3 Yucailu, Guilin, Guangxi, 541004, China) |
| Abstract: |
| The binding of Zn(Ⅱ) to human serum albumin(HSA) and bovine serum albumin(BSA) at physiological (pH7.43) were studied by the method of equilibrium dialysis. The results showed that there were 1.1 strong binding sites and 9.0 weak binding sites of Zn(Ⅱ) in HSA,Their stability constants were 1.04×105 and 1.67×103, respectively. In BSA there were 1.2 strong binding sites and 9.7 weak binding sites of Zn(Ⅱ). Their stability constants were 8.2×104 and 1.9×103, respectively.The Hill coefficients which are 0.87 for Zn(Ⅱ) -HSA,0.84 for Zn(Ⅱ) -BSA demonstrate that the binding of HSA and BSA with Zn(Ⅱ) just produce a little negative cooperativity. |
| Key words: zinc (Ⅱ) serum albumin equilibrium dialysis cooperative effect |
