| 引用本文： |
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涂楚桥,梁宏,王光辉.Ag+离子对漆树漆酶催化活性的抑制作用[J].广西科学,1999,6(2):109-110,114. [点击复制]
- Tu Chuqiao,Liang Hong,Wang Guanghui.Inhibition of the Oxidoreductase Activity of Rhus vernicifera Laccase by Ag+ Ion[J].Guangxi Sciences,1999,6(2):109-110,114. [点击复制]
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| 摘要: |
| 以5,6-二溴-2,3-二氰基氢醌(DDBQH2)为底物,在pH值4.4和30±0.2℃条件下,用分光光度法考察了Ag+离子对漆树漆酶催化活性的影响。结果表明,Ag+离子对DDBQH2的漆酶催化氧化反应有明显的抑制作用,这种作用具有罕见的反竞争性特征,抑制剂常数Ki=(2.1±0.5)×10-5 mol·dm-3。 |
| 关键词: 漆树漆酶 Ag+离子 反竞争性抑制作用 |
| DOI： |
| 投稿时间：1998-05-19修订日期：1998-10-29 |
| 基金项目:广西自然科学基金资助项目。 |
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| Inhibition of the Oxidoreductase Activity of Rhus vernicifera Laccase by Ag+ Ion |
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Tu Chuqiao, Liang Hong, Wang Guanghui
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| (Dept. of Chemistry & Chemical Engineering, Guangxi Normal Univ., 3 Yucailu, Guilin, Guangxi, 541004) |
| Abstract: |
| Kinetic experiments were designed to examine the effects of Ag+ ion-on the oxidation of DDBQH2(5,6-dibromo-2,3-dicyanohydroquinone) catalyzed by Rhus vernicifera laccase. The result was that Ag+ ion-could uncompetitively inhibit the catalytic activity of laccase at pH4.4 and 30±0.2℃,the inhibition constant Ki=(2.1±0.5) ×10-5 mol·dm -3. |
| Key words: Rhus vernicifera laccase Ag (Ⅰ) uncompetitive inhibition |
