| 摘要: |
| 介绍一种以平面热解石墨电极为工作电极,用于识别与计量健康人白细胞的半微分伏安型生物传感器。在本传感器上建立健康人淋巴细胞及粒细胞的识别模式,用本传感器计量细胞数,当活细胞数在(1.8~18.1)×104cels范围内,循环扫描峰电流的半微分值与细胞数之间存在着良好的线性相关(r=0.9888)。 |
| 关键词: 细胞识别 生物传感器 半微分循环伏安法 人白细胞 人粒细胞 人淋巴细胞 |
| DOI： |
| 投稿时间：1998-05-11 |
| 基金项目: |
|
| A Study on the Voltammetric Biosensor for Discriminating and Counting Human Leukocyte |
|
Cai Haobin, Xu Chunxiang
|
| (Biomedical Institute of Guilin Medical College, 8 Pingfenglu, Guilin, Guangxi, 541004) |
| Abstract: |
| A Biosensor which can discriminate and count the human leukocyte is reported. The leukocyte on trap membrane applied to basal plane pyrolytic graphite electrode is detected with semi-differential voltammetry in phosphate buffered solution. The model of discriminating human granulocyte and lymphocyte has been constructed. A good line correlation between cell numbers blocking trap membrane and semi-differential peak current has been obtained in the range of cell numbers from 1.8×104 to 18.1×104 cells (r=0.9888). |
| Key words: cell discrimination biosensor semi-differential voltammetry Human leukocyte Human granulocyte Human lymphocyte |
