| 摘要: |
| 应用聚合酶链反应技术检测缺失型α地中海贫血-2。特异性引物选择性扩增α2和α1基因,PCR产物经琼脂糖凝胶电泳后即可对-α3.7和-α4.2缺失型作出基因诊断。此方法简便,快速和可靠。本研究为在缺失型α地中海贫血-2高发区进行-α3.7和-α4.2缺失型的筛查和产前诊断提供了一个新的方法。 |
| 关键词: 缺失型α地中海贫血-2 -α3.7和-α4.2缺失型 聚合酶链反应(PCR) |
| DOI： |
| 投稿时间：1998-07-31 |
| 基金项目: |
|
| Detection of Common Deletional α-Thalassemia 2 determinants |
|
Chen Ping, Long Guifang
|
| (Guangxi Medical University, 6 Binhulu, Nanning, Guangxi, 530021) |
| Abstract: |
| Using polymerase chain reaction to detect common deletional α-thalassemia-2. Specific primers selectively amplify the α2 and α1 genes. After electrophoresis of PCR products, then can make diagnosis for genotypes of the-α3.7 and α4.2 deletions. This method is simple, fast, reliable and non-radioactive. It offers a new method for large screening and prenatal diagnosis of-α3.7 and-α42 deletions in the area with high frequency of deletional α-thalassemia-2. |
| Key words: deletional α-thalassemia-2 -α3.7 and-α4.2 deletions polymerase chain reaction (PCR) |
