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  • 王家睦,汪一平.棒状杆菌诱发质粒简报[J].广西科学,1997,4(4):316-318,324.    [点击复制]
  • Wang Jiamu,Wang Yiping.An Inducible Plasmid in Corynebacterium[J].Guangxi Sciences,1997,4(4):316-318,324.   [点击复制]
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棒状杆菌诱发质粒简报
王家睦, 汪一平
0
(广西医科大学, 南宁市滨湖路6号 530021)
摘要:
用溴化乙锭诱发质粒,溴化乙锭不同浓度对棒状杆菌基因组起不同作用,低浓度无作用;高浓度消除质粒;适中浓度能诱发质粒。本试验诱发出4种大小不同质粒,转种5代质粒消失。
关键词:  棒状杆菌  质粒  DNA
DOI:
投稿时间:1997-04-09修订日期:1997-08-22
基金项目:广西区科委资助项目。
An Inducible Plasmid in Corynebacterium
Wang Jiamu, Wang Yiping
(Department of Plasmidology, Guangxi Medical University, 6 Binhulu, Nanning, Guangxi, 530021)
Abstract:
A review summarised the plasmids of nonpathogenic Corynebacterium.Corynebacterium pekingense AS 1.299 had plasmids,which could be induced by treatment of ethidium bromide (EB). Their molecular sizes were 36.2×106,15.2×106,13.0×106 and 9.8×106 respectively,compared with 8 known plasmids of E.coliv 517.AS 1.299 strain had no plasmids originally. These inducible plasmids disappeared spontaneously from the host after transfered to fresh media by the fifth time.
Key words:  corynebacterium  plasmid  DNA

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