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麻秀珍,胡刚,唐文红,翁健,韦毅,刘自民.广西地区恒河猴(Rhesus Monkey)B病毒相关抗体的检查研究[J].广西科学,1997,4(2):126-128. [点击复制]
- Ma Xiuzhen,Hu Gang,Tang Wenhong,Weng Jian,Wei Yi,Liu Zimin.On Detecting B Virus Related Antibody in Rhesus Monkey from Guangxi[J].Guangxi Sciences,1997,4(2):126-128. [点击复制]
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| 广西地区恒河猴(Rhesus Monkey)B病毒相关抗体的检查研究 |
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麻秀珍1, 胡刚2, 唐文红3, 翁健4, 韦毅5, 刘自民5
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| (1.中华人民共和国南宁动物检疫所, 南宁市友爱北路51号 530001;2.西南林学院, 云南昆明 650224;3.广西师范大学生物系, 桂林市三里店 541004;4.原广西农业大学附属中学, 南宁市秀灵路13号 530001;5.广西林业厅野生动植物和自然保护区管理站, 南宁市七星路 530022) |
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| 摘要: |
| 采用玻片免疫酶法(IEA)和酶联免疫吸附试验法(ELISA),检查了来自广西不同来源恒河猴B病毒的相关抗体。结果表明:广西野生恒河猴受B病毒的感染较为普遍,来自龙虎山及扶绥保护区的猴,B病毒相关抗体阳性率分别是79.7%和75.3%。来自穿洞河及布柳河保护区的猴,B病毒相关抗体阳性率分别是26.1%和28.9%。大新保护区猴阳性率为57.1%。在不同组猴中,小群关养组猴相关抗体阳性率最高,野生猴次之,自繁猴最低。三组间,猴B病毒相关抗体阳性率的差异极显著(P<0.05),经ELISA检查确定为阴性的猴,用IEA检查出7.5%(15/200)的猴被判定为阳性猴。 |
| 关键词: 恒河猴 B病毒 玻片免疫酶法 酶联免疫吸附试验法 |
| DOI： |
| 投稿时间：1996-03-25修订日期：1997-01-15 |
| 基金项目: |
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| On Detecting B Virus Related Antibody in Rhesus Monkey from Guangxi |
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Ma Xiuzhen1, Hu Gang2, Tang Wenhong3, Weng Jian4, Wei Yi5, Liu Zimin5
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| (1.Nanning Animal Quarantine Service of P. R. China, 51 North You'ailu, Nanning, Guangxi, 530001;2.Southwest China Forestry College, Kunming, Yunnan, 650224;3.Dept. of Biology, Guangxi Normal Univ., Salidian, Guilin, Guangxi, 541004;4.Middle School of Guangxi Agriculture University, 13 Xiulinglu, Nanning, Guangxi, 530001;5.The Wildlife and Natural Reserve Managing Station of Guang xi Department of Forestry, Qixinglu, Na nning, Guangxi, 530022) |
| Abstract: |
| The related antibody to B virus (BVRA) of Rhesus monkey from different regions of Guangxi were detected by IEA and ELISA. The results showed that the wild monkeys were generally infected with B virus.The positive rate of BVRA of the Longhu Monkey was 79.7%, the Fusui was 75.3%, the Daxin was 57.1%,the Chuandonghe was 26.1%, and the Buliuhe was 28.9%.The positive rate of BVRA of the small colony captive group was the highest, following by the wild captive group, and the self reproduction captive group was the lowest.In these three groups,the differences of the positive of BVRA among them were significant (P<0.05). Of the monkeys with negative in B virus related antibody detected by ELISA,there were 7.5% showing positive by IEA. It showed that IEA was better than ELISA in Qurantine work. |
| Key words: Rhesus monkey B virus IEA ELISA |
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