| 引用本文： |
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陈一强,施焕中,柳广南,钟小宁,梁国容,许辉.哮喘患者肺泡巨噬细胞中IL-6mRNA表达的研究[J].广西科学,1996,3(3):51-54. [点击复制]
- Chen Yiqiang,Shi Huangzhong,Liu Guangnan,Zhong Xiaoning,Liang Guorong,Xu Hui.Investigation of Interleukin-6 mRNA Expression in Asthmatic Alveolar Macrophages[J].Guangxi Sciences,1996,3(3):51-54. [点击复制]
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| 摘要: |
| 应用逆转录酶链反应及索氏转移杂交技术分析过敏性支气管哮喘患者(激素治疗组9例,非激素治疗组12例)及正常对照组(10例)肺泡巨噬细胞(alveolarmacrophages,AM)中白细胞介素一6(interleukin-6,IL-6)的mRNA表达。IL-6/β-actin比值表示。结果发现未用激素治疗的哮喘患者AM的IL-6/β-actin为0.66±0.32,显著高于正常对照者(0.34±0.13,P<0.01)及激素治疗组(0.41±0.15,P<0.05)。提示表达IL-6的AM在哮喘的发病机理中可能具有重要的作用,而皮质激素治疗哮喘的机制之一可能与其抑制AM表达及产生IL-6的能力有关。 |
| 关键词: 白细胞介素-6 基因表达 巨噬细胞 哮喘 |
| DOI： |
| 投稿时间：1996-03-24 |
| 基金项目: |
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| Investigation of Interleukin-6 mRNA Expression in Asthmatic Alveolar Macrophages |
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Chen Yiqiang, Shi Huangzhong, Liu Guangnan, Zhong Xiaoning, Liang Guorong, Xu Hui
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| (Department of Respiratory, the First Affliated Hospital of Guangxi Medical University, 6 Binhu Road, Nanning, Guangxi, 530027) |
| Abstract: |
| The interleukin-6 (IL-6) mRNA expression was analyzed in alveolar macrophage (AM) from allergic asthmatics treated with corticosteriod (9 cases) or not (12 cases), as well as normal subjects (10 cases). This was achieved by using reverse transcription-polymerase chain reaction and Southern blots techniques. The result was described by IL-6/β-actin ratio and was found that the x ±s of IL-6/β-actin ratio in AM from asthmatics without corticosteriod treatment was 0.66±0.32, which significantly higher than that from either normal subjects (0.34±0.13, P<0.01) or asthmatics treated with corticosteried (0.41±0.15, P<0.05). These results suggest that AM expression of IL-6 may be important in phathogenesis of human asthma, and that one respect of mechanisms of corticosteriod in asthma treatment may be inhibition of IL-6 and thus supression of inflammation in airway. |
| Key words: interleukin-6 gene expression alveolar macrophage asthma |
