| 引用本文： |
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欧阳权,曾炼武,李洁汉.桉树组培育苗新技术[J].广西科学,1994,1(3):49-57. [点击复制]
- Ouyang Quan,Zhen Lianwu,Li Jiehan.New Techniques of Culturing Eucalyptus Plantlets by Tissue Culture[J].Guangxi Sciences,1994,1(3):49-57. [点击复制]
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| 摘要: |
| 全面介绍桉树组织培养试验研究成果。通过筛选出改良H、B1、B2基本培养基,成功地从桉树愈伤组织诱导发生胚性细胞团,分化出根苗齐全、遗传性状稳定、频率很高的胚状体苗,成苗系数高达1011;用杂交优势种的芽器官离体培养,不经过愈伤组织途径,避免脱分化过程易引起染色体变异的弊端,以芽繁芽,以苗繁苗成功,年繁殖系数3.512,解决了桉树组织培养的一系列技术关键。 |
| 关键词: 桉树 组织培养 胚状体 以芽繁芽 |
| DOI： |
| 投稿时间：1993-11-03 |
| 基金项目: |
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| New Techniques of Culturing Eucalyptus Plantlets by Tissue Culture |
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Ouyang Quan, Zhen Lianwu, Li Jiehan
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| (Forestry Research Institute of Qinzhon Prefecture, Pangqin Road, Qinzhou, Guangxi, 535000) |
| Abstract: |
| The experimental results on tissue culture of Eucalyptus are overall introduced. Through selecting improved H, B1, B2 besic medium, the embryonic cell aggregated were induced successfully from Eucalyplus callus. The embryoid plantlets were differentiated, and with complete root, stable genetic character and high frequency. The coefficient of plantlets can be up to 1011. The method of propagating bud organ of hybrid dominant in vitro (without callus culturing) can be used to avoid chromosome variation during differentiation.Propagating buds by using buds and propagating plantlets by using plantlets were successfully out, and the annual propagation coefficient can be 3.512. A series of technical matters of Eucalyplus tissue culture have been solved. |
| Key words: Eucalyplus tissue culture embryoid propagating buds by using buds |
