| 摘要: |
| 短叶茳芏为我国南方沿海分布面积较大的原生滨海盐沼草本植物，在保滩护岸和改良土壤等领域具有重要的意义。本文通过获取短叶茳芏叶绿体基因组信息特征，探究其基因组特征和及分析物种亲缘关系。利用Illumina高通量测序技术构建了短叶茳芏叶绿体全基因组图谱。结果显示，短叶茳芏叶绿体基因组全长186314bp，为典型的四分体结构；其中大单拷贝区和小单拷贝区大小分别为101162bp和10310bp，反向重复区大小为37421bp，共注释叶绿体基因141个，包括94个蛋白编码基因，39个转运RNA基因和8个核糖体RNA基因。共鉴定出105个SSR位点和77个长重复序列，以及挖掘出183个SNP位点。系统发育分析表明，短叶茳芏与同属莎草属的茳芏、香附子亲缘关系最近。这些结果丰富了莎草科植物的遗传资源，为进一步研究莎草科植物的系统发育提供了分子基础。 |
| 关键词: 短叶茳芏 叶绿体基因组 高通量测序 特征分析 系统发育分析 |
| DOI： |
| 投稿时间：2024-06-30修订日期：2024-10-28 |
| 基金项目: |
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| Assembling and Characteristic Analysis of the Complete Chloroplast Genome of Cyperus malaccensis subsp. monophyllus from High-Throughput Sequencing Data |
|
CHEN KUN, PAN Lianghao, LIAO Xin
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| (Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Academy of Marine Science) |
| Abstract: |
| Cyperus malaccensis subsp. monophyllus is a native salt marsh herb with a large distribution area along the southern coast of China, which is of great significance in the fields of beach protection and soil improvement. In this paper, we obtained the genomic information of the chloroplasts of C. malaccensis subsp. monophyllus, explored its genomic features and analyzed the species affinities. The whole genome of C. malaccensis subsp. monophyllus chloroplast was characterized by Illumina high-throughput sequencing. The results showed that the chloroplast genome of C. malaccensis subsp. monophyllus was 186314 bp in length, with a typical tetrameric structure, in which the size of the large single-copy (LSC) region and the small single-copy(SSC) region were 101162 bp and 10310 bp, respectively, and the size of the inverted repeat(IR) region was 37421 bp, and the total number of annotated chloroplast genes was 141, including 94 protein-coding genes, 39 transporter RNA genes, and 8 ribosome RNA genes. A total of 105 SSR sites and 77 long repeat sequences were identified, and 183 SNP sites were mined. Phylogenetic analyses showed that C. malaccensis subsp. monophyllus was the closest relative to Cyperus malaccensis and Cyperus rotundus, which belong to the same genus of Cyperus. These results enriched the genetic resources of the family Cyperaceae and provided a molecular basis for further studies on the phylogeny of Cyperaceae. |
| Key words: Cyperus malaccensis subsp. Monophyllus Chloroplast genome High-throughput sequencing Characterization Phylogenetic analysis |
