| 摘要: |
| 全球范围的生态系统正面临前所未有的生物多样性丧失和群落结构变化，为及时、准确检测到这些变化，科学家们不断探索新的监测技术。其中，环境DNA（environmental DNA，eDNA）技术作为一种非侵入性的方法已经广泛的应用于生物资源评估和生物多样性调查。然而环境DNA技术存在着一些假阳性的问题影响了监测准确性。相比之下，环境RNA（environmental RNA，eRNA）技术可以更有效地区分活体（代谢活跃）的生物体或死亡（休眠）的生物体，减少假阳性，提高监测可靠性。但环境RNA技术还尚未为人熟知，特别是在国内还极少被应用。本文旨在综述eRNA技术的在生物多样性研究中的现状，通过文献计量学对eRNA在不同水域生态系统中的研究进展进行了分析，并与传统监测方法和eDNA技术进行比较，探讨其优势、限制因素和存在问题。 |
| 关键词: 环境RNA 环境DNA 生物监测 生物多样性 方法学 |
| DOI： |
| 投稿时间：2024-06-03修订日期：2024-10-21 |
| 基金项目:广西科技人才专项【桂科AD20159032】; 广西红树林保护与利用重点实验室基金(GKLMC-22A07) |
|
| Research progress of environmental RNA in biodiversity monitoring |
|
CHEN KUN, QIU SI TING, LIAO XIN
|
| (Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Academy of Marine Science (Guangxi Mangrove Research Center) |
| Abstract: |
| Ecosystems on a global scale are facing unprecedented biodiversity loss and changes in community structure, and scientists are constantly exploring new monitoring techniques to detect these changes in a timely and accurate manner. Among them, environmental DNA (eDNA) technology, as a non-invasive method, has been widely used in biological resource assessment and biodiversity surveys. However, the environmental DNA technique has some false-positive problems that affect the accuracy of monitoring. In contrast, environmental RNA (eRNA) technology can more effectively distinguish living (metabolically active) organisms from dead (dormant) organisms, reducing false positives and improving the reliability of monitoring. However, environmental RNA technology is not yet well known, especially in China, where it is rarely used. The purpose of this paper is to review the current status of eRNA technology in biodiversity research, analyze the research progress of eRNA in different aquatic ecosystems through bibliometrics, compare it with traditional monitoring methods and eDNA technology, and explore its advantages, limitations and problems. |
| Key words: environmental RNA environmental DNA biological monitoring biodiversity methodological |
