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熊向英,曾梦清,蒋艳,董兰芳,赖俊翔,陈瑞芳.织锦巴非蛤育苗期水体菌群结构特征[J].广西科学院学报,2024,40(1):58-65. [点击复制]
- XIONG Xiangying,ZENG Mengqing,JIANG Yan,DONG Lanfang,LAI Junxiang,CHEN Ruifang.Structure Characteristics of the Water Microbial Community during Seedling Stage of Paphia textile Larvae[J].Journal of Guangxi Academy of Sciences,2024,40(1):58-65. [点击复制]
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| 织锦巴非蛤育苗期水体菌群结构特征 |
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熊向英1,2,3, 曾梦清2, 蒋艳1,2,3, 董兰芳1,2, 赖俊翔1,3, 陈瑞芳1,2,3
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| (1.广西科学院, 广西海洋科学院(广西红树林研究中心), 广西近海海洋环境科学重点实验室, 广西南宁 530007;2.广西海洋研究所有限责任公司, 广西海水养殖新品种繁育工程技术研究中心, 广西北海 536000;3.北部湾海洋产业研究院, 广西防城港 538000) |
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| 摘要: |
| 为探究织锦巴非蛤(Paphia textile)幼体发育各阶段水体菌群的组成和结构特征，为其苗种规模化人工繁育提供理论依据，本研究采用高通量测序技术对幼体在D形幼虫期(HD)、壳顶期(HK)、变态前期(HB)、变态后期(HA)、稚贝期(HZ)的水体菌群进行测序分析。Alpha多样性指数结果表明，HD菌群丰度和菌群多样性均最高，而HZ菌群丰度最低，HB菌群多样性最低。在门分类水平上，幼体发育所有阶段中占优势的细菌门类主要有变形菌门(Proteobacteria)[包括α-变形菌纲(Alphaproteobacteria)和γ-变形菌纲(Gammaproteobacteria)]、拟杆菌门(Bacteroidota)和放线菌门(Actinobacteriota)。在科分类水平上，红杆菌科(Rhodobacteraceae)和黄杆菌科(Flavobacteriaceae)是整个育苗周期的主导类群；微杆菌科(Microbacteriaceae)相对丰度在HB低于1%，在HA和HZ逐渐增长，分别为9.95%和12.82%；冷形菌科(Cryomorphaceae)相对丰度在HD不足1%，在HK、HA和HZ的相对丰度较高，为5.98%-7.67%。值得注意的是，海源菌科(Idiomarinceae)的相对丰度在织锦巴非蛤幼体发育不同阶段有较大差异，在HD和HK的相对丰度不足1%，但在HB迅速增加到31.90%。综上，织锦巴非蛤幼体不同发育阶段水体菌群多样性和组成发生了明显变化。 |
| 关键词: 织锦巴非蛤 育苗期 水体菌群 细菌多样性 高通量测序 |
| DOI：10.13657/j.cnki.gxkxyxb.20240319.007 |
| 投稿时间：2023-12-04修订日期：2023-12-29 |
| 基金项目:国家重点研发计划“海洋农业与淡水渔业科技创新”重点专项(2022YFD2401200);广西科学院创新团队启动经费(CQ-D-2410)和广西自然科学基金项目(2020GXNSFBA159011) |
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| Structure Characteristics of the Water Microbial Community during Seedling Stage of Paphia textile Larvae |
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XIONG Xiangying1,2,3, ZENG Mengqing2, JIANG Yan1,2,3, DONG Lanfang1,2, LAI Junxiang1,3, CHEN Ruifang1,2,3
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| (1.Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Science (Guangxi Mangrove Research Center), Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Guangxi Engineering Technology Research Center of Breeding of New Mariculture Varieties, Guangxi Institute of Oceanology Co., Ltd., Beihai, Guangxi, 536000, China;3.Beibu Gulf Marine Industry Research Institute, Fangchenggang, Guangxi, 538000, China) |
| Abstract: |
| In order to explore the composition and structure characteristics of water microbial community in Paphia textile larvae at different developmental stages,and to provide a theoretical basis for large-scale artificial breeding of seedlings,high-throughput sequencing technology was used to sequence and analyze the water microbial community of larvae at D-shaped larval stage (HD),shell top stage (HK),pre-metamorphosis stage (HB),post metamorphosis stage (HA) and juvenile shell stage (HZ).The results of Alpha diversity index showed that the abundance and diversity of microbial communities in HD were the highest,while the abundance of microbial communities in HZ was the lowest and the diversity of microbial communities in HB was the lowest.At the phylum level,the dominant bacterial communities in all stages of larval development were Proteobacteria [including Alphaproteobacteria and Gammaproteobacteria],Bacteroidota and Actinobacteriota.At the family level,the Rhodobacteriaceae and Flavobacteriaceae were the dominant groups in the whole breeding cycle.The relative abundance of Microbacteriaceae was less than 1% in HB,and gradually increased in HA and HZ,which were 9.95% and 12.82%,respectively.The relative abundance of Cryomorphaceae was less than 1% in HD,and the relative abundance in HK,HA and HZ was higher,which was 5.98%-7.67%.It is worth noting that the relative abundance of Idiomarinceae had significant differences at different stages of P.textile larval development.The relative abundance of Idiomarinceae in HD and HK was less than 1%,but increased rapidly to 31.90% in HB.In summary,the diversity and composition of water microbial community in different developmental stages of P.textile larvae changed significantly. |
| Key words: Paphia textile seedling stage water microbial community bacterial diversity high-throughput sequencing |
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