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陈建荣,徐雨茶,阳丽艳,黄艳冰,杨登峰,潘丽霞.几丁质降解菌的筛选及其降解虾壳废弃物的研究[J].广西科学院学报,2023,39(4):452-460. [点击复制]
- CHEN Jianrong,XU Yucha,YANG Liyan,HUANG Yanbing,YANG Dengfeng,PAN Lixia.Screening of Chitin-degrading Bacteria and the Study of Their Efficient Degradation of Shrimp Shell Waste[J].Journal of Guangxi Academy of Sciences,2023,39(4):452-460. [点击复制]
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| 几丁质降解菌的筛选及其降解虾壳废弃物的研究 |
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陈建荣1, 徐雨茶1, 阳丽艳1, 黄艳冰2, 杨登峰2, 潘丽霞1
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| (1.广西科学院, 非粮生物质能技术全国重点实验室, 广西南宁 530007;2.广西科学院, 广西海洋科学院(广西红树林研究中心), 广西海洋天然产物与组合生物合成化学重点实验室, 广西南宁 530007) |
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| 摘要: |
| 虾蟹壳废弃物产量的日益增加造成了巨大的资源浪费和严重的环境污染。为了提高虾蟹壳废弃物的综合利用价值，本研究从土壤中筛选出几丁质高效降解菌，并通过单因素试验探究降解虾壳的最佳条件及其对虾壳的降解能力。利用胶体几丁质为唯一碳源，采用平板透明圈法对来源于喀斯特地貌的土壤微生物进行富集、分离和纯化，获得了4株具有几丁质降解活力的菌株:粘质沙雷氏菌Serratia marcescens gxas3、蜡样芽孢杆菌Bacillus cereus gxas5、嗜麦芽糖窄食单胞菌Stenotrophomonas maltophilia gxas7以及水生几丁质降解菌Chitinilyticum aquatile gxas1。其中C. aquatile gxas1在96 h时达到最大粗酶活力0.244 U/mL，优于其他3株菌株。利用单因素试验探究C. aquatile gxas1降解虾壳废弃物的能力，并优化其培养基和培养条件，最终确定最佳产酶培养基为果糖2 g/L、KH2PO4 0.7 g/L、K2HPO4 0.3 g/L、MgSO4·6H2O 0.6 g/L、酵母粉0.5 g/L、牛肉膏4 g/L、蛋白胨4 g/L。最佳发酵条件为接种量2.0%、pH值8.0、温度20 ℃、发酵时间9 d。在最佳发酵条件下，C. aquatile gxas1对虾壳废弃物达到最大降解率60%，其中脱蛋白率为76.77%，几丁质回收率为87%。该研究为微生物降解虾蟹壳废弃物提供了重要的理论依据。 |
| 关键词: 微生物降解 几丁质 虾蟹壳废弃物 几丁质降解菌 水生几丁质菌gxas1 |
| DOI：10.13657/j.cnki.gxkxyxb.20231226.012 |
| 投稿时间：2023-09-11修订日期：2023-10-07 |
| 基金项目:国家自然科学基金项目(31960203)，广西科技基地和人才专项(桂科AD23023007)和广西创新驱动发展专项重大科技创新基地建设项目“非粮生物质酶解国家重点实验室”(2022-36-Z06)资助。 |
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| Screening of Chitin-degrading Bacteria and the Study of Their Efficient Degradation of Shrimp Shell Waste |
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CHEN Jianrong1, XU Yucha1, YANG Liyan1, HUANG Yanbing2, YANG Dengfeng2, PAN Lixia1
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| (1.National Key Laboratory of Non-food Biomass Energy Technology, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Marine Sciences (Guangxi Mangrove Research Center), Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| The increasing production of shrimp and crab shell waste has caused huge waste of resources and serious environmental pollution.In order to improve the comprehensive utilization value of shrimp and crab shell waste,this study screened chitin-degrading bacteria from soil,and explored the optimal conditions for degrading shrimp shells and the degradation ability of shrimp shells through single factor experiments.Using colloidal chitin as the sole carbon source,the soil microorganisms derived from karst landform was enriched,separated and purified by plate transparent circle method.Four strains with chitin-degrading activity were obtained:Serratia marcescens gxas3,Bacillus cereus gxas5,Stenotrophomonas maltophilia gxas7 and Chitinilyticum aquatile gxas1.Among them,C. aquatile gxas1 reached the maximum crude enzyme activity of 0.244 U/mL at 96 h,which was better than the other three strains.The ability of C. aquatile gxas1 to degrade shrimp shell waste was investigated by single factor experiment,and its medium and culture conditions were optimized.Finally,the optimal enzyme production medium was determined to be fructose 2 g/L,KH2PO4 0.7 g/L,K2HPO4 0.3 g/L,MgSO4·6H2O 0.6 g/L,yeast powder 0.5 g/L,beef extract 4 g/L,peptone 4 g/L.The optimum fermentation conditions were inoculation amount of 2.0%,pH value of 8.0,temperature of 20 ℃ and fermentation time of 9 d.Under the optimal fermentation conditions,the maximum degradation rate of C. aquatile gxas1 shrimp shell waste reached 60%,the deproteinization rate was 76.77%,and the chitin recovery rate was 87%.This study provides an important theoretical basis for microbial degradation of shrimp and crab shell waste. |
| Key words: biodegradation chitin shrimp and crab shell waste chitin-degraded bacteria Chitinilyticum aquatile gxas1 |
