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杨一山,柴胜丰,唐健民,罗亚进,杨秀星,韦霄.鹅毛玉凤花总多糖提取工艺优化及抗氧化活性研究[J].广西科学院学报,2022,38(2):188-196. [点击复制]
- YANG Yishan,CHAI Shengfeng,TANG Jianmin,LUO Yajin,YANG Xiuxing,WEI Xiao.Study on Extraction Process Optimization of Total Polysaccharides from Habenaria dentata and Its Antioxidant Activity[J].Journal of Guangxi Academy of Sciences,2022,38(2):188-196. [点击复制]
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| 鹅毛玉凤花总多糖提取工艺优化及抗氧化活性研究 |
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杨一山1,2, 柴胜丰2, 唐健民2, 罗亚进3, 杨秀星3, 韦霄2
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| (1.桂林医学院药学院, 广西桂林 541004;2.广西壮族自治区中国科学院广西植物研究所, 广西植物功能物质研究与利用重点实验室, 广西桂林 541006;3.广西雅长兰科植物国家级自然保护区管理中心, 广西百色 533209) |
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| 摘要: |
| 本研究以药用植物鹅毛玉凤花(Habenaria dentata)的块茎为材料，在单因素试验的基础上，选取提取时间、料液比和提取温度进行响应面试验，优化鹅毛玉凤花总多糖的提取工艺，并通过测定总多糖的DPPH自由基(DPPH·)清除率、羟基自由基(·OH)清除率、超氧阴离子自由基(O2-·)清除率和总还原力,探究鹅毛玉凤花的体外抗氧化活性。结果表明，鹅毛玉凤花总多糖的最佳提取条件为提取时间7 h，料液比1∶58.7 (g/mL)，提取温度73.49℃，优化验证后的鹅毛玉凤花总多糖得率为9.59%，相对标准偏差(RSD)为0.13%。抗氧化试验结果表明，鹅毛玉凤花总多糖对DPPH·、·OH、O2-·的半抑制浓度(IC50)分别为1.385 mg/mL、0.006 mg/mL和0.020 mg/mL，其总还原力是L-抗坏血酸的2.24%，表明鹅毛玉凤花总多糖具有较好的抗氧化能力。 |
| 关键词: 鹅毛玉凤花|总多糖|单因素试验|正交设计|抗氧化活性 |
| DOI：10.13657/j.cnki.gxkxyxb.20220622.011 |
| 投稿时间：2022-01-06 |
| 基金项目:广西林业科技推广示范项目(桂林科字[2021]第28号)和广西植物功能物质研究与利用重点实验室项目(ZRJJ2020-6,ZRJJ2020-9)资助。 |
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| Study on Extraction Process Optimization of Total Polysaccharides from Habenaria dentata and Its Antioxidant Activity |
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YANG Yishan1,2, CHAI Shengfeng2, TANG Jianmin2, LUO Yajin3, YANG Xiuxing3, WEI Xiao2
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| (1.College of Pharmacy, Guilin Medical University, Guilin, Guangxi, 541004, China;2.Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Siences, Guilin, Guangxi, 541006, China;3.Guangxi Yachang Orchid National Nature Reserve Management Center, Baise, Guangxi, 533209, China) |
| Abstract: |
| In this study,the tuber of the medicinal plant Habenaria dentata was selected as the material. On the basis of single-factor experiments,extraction time,solid-liquid ratio and extraction temperature were selected for response surface test to optimize the extraction process of total polysaccharides from H.dentata. And the in vitro antioxidant activity of H.dentata was studied by determining DPPH radical scavenging activities (DPPH·),hydroxyl radical scavenging activities (·OH),superoxide anion radical scavenging activities (O2-·) and total reducing ability of total polysaccharides. The results showed that the optimum extraction conditions of total polysaccharides from H.dentata were as follows:The extraction time was 7 h,the solid-liquid ratio was 1∶58.7 (g/mL),and the extraction temperature was 73.49℃,the optimized validated of total polysaccharide yield from H.dentata was 9.59%,and the Relative Standard Deviation (RSD) was 0.13%. Antioxidant test results showed that the half maximal inhibitory concentration (IC50) of total polysaccharides on DPPH·,hydroxyl radicals (·OH) and superoxide anions (O2-·) were 1.385 mg/mL,0.006 mg/mL and 0.020 mg/mL,respectively. The total reducing ability was 2.24% of L-ascorbic acid,indicating that total polysaccharides had good antioxidant capacity. |
| Key words: Habenaria dentate|total polysaccharide|single factor experiment|orthogonal design|antioxidant activity |
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