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邹蓉,孙菲菲,邱德全,蒋运生,蒙美清,梁敏玲.通脱木黄酮提取工艺及抗氧化活性研究[J].广西科学院学报,2022,38(1):45-52,60. [点击复制]
- ZOU Rong,SUN Feifei,QIU Dequan,JIANG Yunsheng,MENG Meiqing,LIANG Minling.Study on Extraction Technology and Antioxidant Activity of Flavonoids from Tetrapanax papyrifer[J].Journal of Guangxi Academy of Sciences,2022,38(1):45-52,60. [点击复制]
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| 通脱木黄酮提取工艺及抗氧化活性研究 |
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邹蓉1, 孙菲菲1,2, 邱德全1,3, 蒋运生1, 蒙美清1,3, 梁敏玲1,3
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| (1.广西壮族自治区中国科学院广西植物研究所, 广西植物功能物质与资源持续利用重点实验室, 广西桂林 541006;2.桂林医学院药学院, 广西桂林 541199;3.百色学院农业与食品工程学院, 广西百色 533000) |
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| 摘要: |
| 本研究利用单因素试验和响应面试验探究超声波法辅助提取通脱木Tetrapanax papyrifer黄酮的最佳工艺，在此基础上分别对采自广西壮族自治区百色市乐业县拉雅、拉雅二沟和河池市南丹县峨嵋村3个地区的通脱木茎髓、叶的黄酮含量进行测定分析，并研究黄酮体外抗氧化活性。结果表明：通脱木黄酮在超声波功率300 W、频率40 kHz条件下，最佳提取条件为料液比1∶50 (g/mL)，乙醇浓度20%，提取温度70℃，提取时间70 min，黄酮提取率为1.62%。拉雅二沟黄酮含量和抗氧化能力均高于其他两个地区，其对·OH、DPPH·、O2-·的消除率分别为19.35%、63.21%、55.65%,同时该地区通脱木茎髓中的黄酮含量显著高于叶子。本研究为通脱木黄酮资源的开发利用提供了数据参考。 |
| 关键词: 通脱木 黄酮提取 响应面法 抗氧化性 超声波辅助提取 |
| DOI：10.13657/j.cnki.gxkxyxb.20220421.002 |
| 投稿时间：2021-06-17 |
| 基金项目:河池科技计划项目(河科AB198807，河科AB210306)和广西植物研究所基本业务费项目(桂植业19002)资助。 |
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| Study on Extraction Technology and Antioxidant Activity of Flavonoids from Tetrapanax papyrifer |
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ZOU Rong1, SUN Feifei1,2, QIU Dequan1,3, JIANG Yunsheng1, MENG Meiqing1,3, LIANG Minling1,3
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| (1.Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China;2.College of Pharmacy, Guilin Medical University, Guilin, Guangxi, 541199, China;3.College of Agriculture and Food Engineering, Baise University, Baise, Guangxi, 533000, China) |
| Abstract: |
| In this study, the single factor test and response surface test were used to explore the optimal extraction process of ultrasonic assisted extraction of the flavonoids from Tetrapanax papyrifer.On this basis, the content of flavonoids in the stems pith and leaves of the three areas of Laya, Laya Ergou, Leye County, Baise City, and Emei Village, Nandan County, Hechi City, Guangxi Zhuang Autonomous Region, China, were determined and analyzed, and the antioxidant activity of flavonoids in vitro was studied.The results showed that under the conditions of ultrasonic power of 300 W and frequency of 40 kHz, the optimal extraction conditions were as follows:Material-liquid ratio of 1∶50 (g/mL), ethanol concentration of 20%, extraction temperature of 70℃, and extraction time of 70 min.The extraction rate of flavonoids was 1.62%.The flavonoids content and antioxidant capacity of Laya Ergou were higher than those of the other two regions, and the elimination rates of ·OH, DPPH·, O2-· were 19.35%, 63.21%, 55.65%, respectively.At the same time, the flavonoids content in stem pith of T.papyrifer was significantly higher than that in leaves.This study provides a data reference for the development and utilization of the flavonoids resources of T.papyrifer. |
| Key words: Tetrapanax papyrifer flavonoid extraction response surface method antioxidant ultrasonic assisted extraction |
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