| 引用本文： |
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廖馨,杨明柳.基于环境DNA的合浦榄根村退化红树林底栖动物多样性的研究[J].广西科学院学报,2021,37(3):288-298. [点击复制]
- LIAO Xin,YANG Mingliu.Study of Benthos Biodiversity in Degraded Mangrove of Lan'gen Village, Hepu, Based on Environmental DNA[J].Journal of Guangxi Academy of Sciences,2021,37(3):288-298. [点击复制]
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| 摘要: |
| 本研究基于环境DNA研究合浦榄根村红树林底栖生物的多样性和生物量，探究因海岸工程造成红树林退化后的底栖生物的变化。实验设置死亡红树林、严重退化红树林（以上合称退化红树林）、尚存活红树林以及对照组红树林4种样地，提取红树林沉积物环境总DNA，并设计特异性引物，利用荧光定量PCR法比较优势物种的相对生物量差异；高通量测序后通过生物信息学分析各样地的物种组成结构。定量实验结果显示，退化红树林中两种甲壳类动物生物量相对较低，两种贝类的生物量在各样地没有显著差异。基于高通量测序的环境DNA揭示死亡红树林、严重退化红树林、尚存活红树林以及对照组红树林的群落结构、优势物种存在差异；退化红树林中，软体动物门丰度减少，环节动物门和刺胞动物门增多。聚类结果显示，尚存活红树林与对照组红树林的群落结构相关性最近，随后是严重退化红树林，死亡红树林的相关性最远。本研究结果反映海岸工程造成退化红树林中底栖动物生物多样性和生物量均产生了变化，同时证明环境DNA是研究红树林大型底栖动物生物多样性和生物量变化的有效手段。 |
| 关键词: 环境DNA 海岸工程 生物多样性 生物量 底栖动物 |
| DOI：10.13657/j.cnki.gxkxyxb.20210928.007 |
| 投稿时间：2021-06-07 |
| 基金项目:合浦县林业局项目（GXZC2020-J3-HXHP-001），广西科技基地和人才专项（桂科AD20159032）和广西红树林保护与利用重点实验室开放基金项目（GKLMC-202102）资助。 |
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| Study of Benthos Biodiversity in Degraded Mangrove of Lan'gen Village, Hepu, Based on Environmental DNA |
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LIAO Xin, YANG Mingliu
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| (Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai, Guangxi, 536000, China) |
| Abstract: |
| In this study, the biodiversity and biomass of benthos in mangrove of Lan'gen Village, Hepu County were studied based on environmental DNA to reveal the changes of benthos after mangrove degradation due to coastal engineering. The total environmental DNA of mangrove sediment was extracted from four sample plots of dead mangroves, severely degraded mangroves (the above collectively referred to degraded mangroves), surviving mangroves and control group mangroves. Specific primers were designed, and the relative biomass differences of dominant species were compared by fluorescence quantitative PCR. After high-throughput sequencing, the species composition structure of each plot was analyzed by bioinformatics. Quantitative experimental results showed that the biomass of two crustaceans in degraded mangroves was relatively low, and there was no significant difference in biomass between the two kinds of mollusks. Environmental DNA based on high-throughput sequencing revealed that there were differences in community structure and dominant species among dead mangroves, severely degraded mangroves, surviving mangroves and control group mangroves. In degraded mangroves, the abundance of Mollusks decreased, while the number of Annelida and Cnidaria increased. Clustering results showed that the community structure of the surviving mangroves had the closest correlation with that of the control group mangroves, followed by that of the severely degraded mangroves, and the community structure of the dead mangroves had the farthest correlation. The results of this study reflected that the biodiversity and biomass of benthos in degraded mangroves caused by coastal engineering had changed, and it proved that environmental DNA was an effective means to study the biodiversity and biomass changes of macro-benthos in mangroves. |
| Key words: environmental DNA coastal engineering biodiversity biomass benthos |
