| 引用本文： |
-
田翠翠,周伟,胡传明,邓银银,杨立恩,许广平.光照强度对条斑紫菜自由丝状体营养藻丝生长及生理指标的影响[J].广西科学院学报,2021,37(1):53-59. [点击复制]
- TIAN Cuicui,ZHOU Wei,HU Chuangming,DENG Yinyin,YANG Li'en,XU Guangping.Effect of Light Intensity on the Growth and Physiological Properties of Free-living Conchocelis of Pyropia yezoensis[J].Journal of Guangxi Academy of Sciences,2021,37(1):53-59. [点击复制]
|
|
| 摘要: |
| 为探究条斑紫菜自由丝状体营养藻丝扩增的适宜光照条件，本研究以条斑紫菜新品种“苏通1号”自由丝状体营养藻丝为材料，研究不同光照强度（5，15，30，45，60，75 μmol·m-2·s-1）对营养藻丝生长及生理特性的影响。结果显示，经25 d培养后，条斑紫菜自由丝状体营养藻丝在15 μmol·m-2·s-1光照强度下生长较好，特定生长率可达3.68%-8.23%，藻体色泽呈暗黑色，藻丝粗壮且长，呈健康状态。藻体叶绿素a、藻红蛋白、藻蓝蛋白含量总体上随光照强度增加表现出先增加后降低趋势，其中在15 μmol·m-2·s-1光照强度下含量最高；类胡萝卜素含量则在各组间差异不显著。藻体光系统PSⅡ最大量子效率Fv/Fm值在15 μmol·m-2·s-1光照强度下最大，约为75 μmol·m-2·s-1组的1.6倍。以上结果表明，15 μmol·m-2·s-1光照强度最有利于条斑紫菜自由丝状体营养藻丝的快速生长。 |
| 关键词: 条斑紫菜 自由丝状体 光照强度 生长 生理指标 |
| DOI：10.13657/j.cnki.gxkxyxb.20210429.009 |
|
| 基金项目:国家重点研发计划“蓝色粮仓科技创新”专项（2018YFD0901502-3，2018YFD0901506-3），国家藻类体系（CARS-50），江苏现代农业（紫菜）产业技术体系项目（JATS[2019] 393，JATS[2019]394，JATS[2019]395）和南通市项目（JCZ20047）资助。 |
|
| Effect of Light Intensity on the Growth and Physiological Properties of Free-living Conchocelis of Pyropia yezoensis |
|
TIAN Cuicui1,2, ZHOU Wei1, HU Chuangming1, DENG Yinyin1, YANG Li'en1, XU Guangping1
|
| (1.Jiangsu Marine Fisheries Research Institute, Nantong, Jiangsu, 226007, China;2.Protection and Utilization of Agricultural Germplasm Resources in Jiangsu Province, Nanjing, Jiangsu, 210014, China) |
| Abstract: |
| In order to explore the suitable light conditions for the amplification of free-living conchocelis of Pyropia yezoensis, the effects of varied light intensities (5, 15, 30, 45, 60 or 75 μmol·m-2·s-1) using LED on the growth and physiological properties of Sutong No.1 were studied, which was a new cultivar of P. yezoensis.The results showed that after 25 d of culturing, under the condition of 15 μmol·m-2·s-1, the free-living conchocelis of P. yezoensis grew well, and the specific growth rate could reach 3.68%-8.23%.The algae color was dark black and conchocelis were thick and long, showing a healthy state.The contents of chlorophyll a, phycocyanin (PC) and phycoerythrin (PE) increased first and then decreased with the increase of light intensity, among which the content was the highest under the light intensity of 15 μmol·m-2·s-1.The carotenoid content was not significantly different among groups.The maximum photon quantum yield of photosystemⅡ(Fv/Fm) was the highest under the light intensity of 15 μmol·m-2·s-1, which was about 1.6 times that of 75 μmol·m-2·s-1 group.In summary, 15 μmol·m-2·s-1 light intensity was most conducive to the rapid proliferation of free-living conchoclis of P. yezoensis. |
| Key words: Pyropia yezoensis free-living conchocelis light intensity growth physiological properties |
