| 引用本文： |
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张红岩,莫勇生,欧娜,谢唯,申乃坤.绿桐增殖、生根培养及炼苗移栽研究[J].广西科学院学报,2020,36(4):411-418. [点击复制]
- ZHANG Hongyan,MO Yongsheng,OU Na,XIE Wei,SHEN Naikun.Study on Multiplication,Rooting and Transplanting of Tissue Culture Plantlets of Paulownia fortunei×Paulownia tomentosa[J].Journal of Guangxi Academy of Sciences,2020,36(4):411-418. [点击复制]
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| 摘要: |
| 本研究旨在建立适于绿桐（Paulownia fortunei×Paulownia tomentosa）的快速无性繁殖技术，为试管苗工厂化生产提供技术参考。实验以绿桐当年生枝条为外植体，对消毒方式、取材部位、植物生长调节剂及其浓度配比条件进行优化，筛选合适的诱导、增殖和生根培养基。实验结果表明，最佳消毒方式为依次采用75%乙醇、等体积的10%次氯酸钠与0.1%升汞混合液、0.1%升汞消毒；当年生嫩条顶端10 cm长度的茎段为最佳外植体；外植体在MS+1.0 mg/L 6-苄氨基嘌呤（6-BA）+1.0 mg/L 2，4-对氯苯氧乙酸（2，4-D）+0.8 mg/L赤霉素（GA3）的培养基中，能诱导出长势健壮的不定芽；在MS+0.8 mg/L 6-BA+0.4 mg/L萘乙酸（NAA）的增殖培养基中，增殖系数达2.02；而生根以1/2 MS+0.6 mg/L吲哚-3-丁酸（IBA）+0.4 mg/L NAA培养基为最佳，生根率达100%；对组培苗进行炼苗后覆膜保湿，移栽存活率可达92%。本研究获得了适合绿桐快速繁殖的培养基，建立了一套绿桐快速繁殖体系。 |
| 关键词: 绿桐 无性繁殖 组织培养 外植体 不定芽 |
| DOI：10.13657/j.cnki.gxkxyxb.20210121.001 |
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| 基金项目:广西科学院基本业务费项目（2017YJJ23016）资助。 |
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| Study on Multiplication,Rooting and Transplanting of Tissue Culture Plantlets of Paulownia fortunei×Paulownia tomentosa |
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ZHANG Hongyan1,2, MO Yongsheng1, OU Na1, XIE Wei1, SHEN Naikun2
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| (1.Institute of Biology Research, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.College of Marine and Biotechnology, Guangxi University for Nationalities, Nanning, Guangxi, 530006, China) |
| Abstract: |
| The purpose of this research is to establish a rapid asexual reproduction technology suitable for Paulownia fortunei×Paulownia tomentosa,and provide a technical reference for the industrial production of test-tube seedlings.In the experiment,the original branches of P.fortunei×P.tomentosa were used as explants,and the disinfection method,the material location,the plant growth regulator and its concentration ratio were optimized,and the appropriate induction,proliferation and rooting medium were screened.The results showed that the best disinfection method was to use 75% ethanol,an equal volume of 10% sodium hypochlorite and 0.1% mercury mixture,and 0.1% mercury liters in sequence.The best explant was the 10 cm long stem at the top of the tender strip.The explants were put in MS+1.0 mg/L 6-benzylaminopurine (6-BA)+1.0 mg/L 2,4-p-chlorobenzene oxyacetic acid (2,4-D) + 0.8 mg/L gibberellin (GA3) medium,which could induce vigorous adventitious buds.In the proliferation medium of MS+0.8 mg/L 6-BA+0.4 mg/L naphthalene acetic acid (NAA),the proliferation coefficient reached 2.02.For rooting,1/2 MS+0.6 mg/L indole-3-butyric acid (IBA) +0.4 mg/L NAA was the best medium,and the rooting rate reached 100%.After the tissue cultured seedlings were hardened and covered with a plastic film to protect moisture,the survival rate could reach 92%.In this study,a medium suitable for the rapid propagation of P.fortunei×P.tomentosa was obtained,and a set of fast-growing propagation system of P.fortunei×P.tomentosa was established. |
| Key words: Paulownia fortunei×Paulownia tomentosa asexual propagation tissue culture explants adventitious bud |
