| 引用本文： |
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覃香香,周玉恒,陈海珊,蔡爱华,王磊,张厚瑞.生物脱毒改善蔗渣水解物木糖醇发酵性能的研究[J].广西科学院学报,2020,36(3):344-351. [点击复制]
- QIN Xiangxiang,ZHOU Yuheng,CHEN Haishan,CAI Aihua,WANG Lei,ZHANG Hourui.Study on Biodetoxification to Improve the Fermentation Performance of Bagasse Hydrolysate Xylitol[J].Journal of Guangxi Academy of Sciences,2020,36(3):344-351. [点击复制]
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| 摘要: |
| 为提高热带假丝酵母Candida troplicalis 1-18代谢产物木糖醇的产量，本研究以蔗渣半纤维素稀酸水解物作为研究材料，以生物脱毒菌株东方伊萨酵母Issatchenkia orientalis S-7细胞干重、水解物在280 nm处紫外吸收物含量、木糖醇产量作为评价指标，考察尿素、pH值、脱毒时间、预浓缩比例和细胞循环利用等5个因素对蔗渣半纤维素稀酸水解物木糖醇发酵性能的影响。结果表明，蔗渣半纤维素稀酸水解物采用Ca（OH）2调节pH值为5，预浓缩至1/2体积，添加2 g·L-1尿素，东方伊萨酵母S-7生物脱毒40 h，脱毒后水解物预浓缩至木糖浓度为150 g·L-1时，热带假丝酵母1-18发酵的木糖醇产量最高，达134.5 g·L-1，比对照组高出70 g·L-1；循环利用细胞进行生物脱毒，脱毒时间缩短13 h；循环利用热带假丝酵母1-18对生物脱毒水解物进行产醇发酵，木糖醇产量达163.8 g·L-1，生成速率为5.85 g·L-1·h-1，比第一代发酵（1.95 g·L-1·h-1）提高2倍。生物脱毒是改善木质纤维稀酸水解物木糖醇发酵性能的有效手段。 |
| 关键词: 东方伊萨酵母 蔗渣 半纤维素水解物 生物脱毒 木糖醇发酵 |
| DOI：10.13657/j.cnki.gxkxyxb.20201027.014 |
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| 基金项目:广西植物功能物质研究与利用重点实验室主任基金项目（ZRJJ2016-18），广西植物研究所基本业务费项目（桂植业18012），广西自然科学基金项目（2018GXNSFAA050093）和广西科技重大专项（桂科AA18118044）资助。 |
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| Study on Biodetoxification to Improve the Fermentation Performance of Bagasse Hydrolysate Xylitol |
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QIN Xiangxiang, ZHOU Yuheng, CHEN Haishan, CAI Aihua, WANG Lei, ZHANG Hourui
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| (Guangxi Lignocellulose Biorefinery Research Center of Engineering Technology, Guangxi Key Laboratory of Functional and Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China) |
| Abstract: |
| To increase the production of xylitol metabolites of Candida troplicalis 1-18,this study used bagasse hemicellulose dilute acid hydrolysate as the research material. And the growth of the biological detoxification strain Issatchenkia orientalis S-7 cells,the 280 nm ultraviolet absorber of the hydrolysate and the yield of xylitol were used as evaluation indicators to investigate the effects of five factors such as urea,pH value,detoxification time,pre-concentration ratio and cell recycling on the fermentation performance of bagasse dilute acid hemicellulose hydrolysate xylose. The results show that the bagasse hemicellulose dilute acid hydrolysate is adjusted to pH=5 with calcium hydroxide,pre-concentrated to 1/2 volume,and 2 g·L-1 urea is added,and I.orientalis S-7 is biologically detoxified for 40 h. When the detoxified hydrolysate is pre-concentrated to the xylose concentration of 150 g·L-1,the xylitol yield of fermentation metabolites in C.troplicalis 1-18 is up to 134.5 g·L-1,which is 70 g·L-1 more than the control group. Recycling cells for biological detoxification shortens the detoxification time by 13 h. Recycling C.troplicalis 1-18 for xylitol production and fermentation of biological detoxification hydrolysates,the xylitol output reaches 163.8 g·L-1,and the generation rate is 5.85 g·L-1·h-1,which is 2 times higher than the first generation (1.95 g·L-1·h-1).Biological detoxification is an effective method to improve the xylitol fermentation performance of lignocellulose dilute acid hydrolysate. |
| Key words: Issatchenkia orientalis bagasse hemicellulose hydrolysate bio-detoxification xylitol fermentation |
