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牛大力内生细菌的多样性及其促生特性研究
苏坤1, 黄英1, 李良波2, 利达朝3, 唐辉4, 黄荣韶1,2
0
(1.广西大学农学院;2.广西中医药大学药学院;3.广西广泽健康产业股份有限公司;4.广西壮族自治区中国科学院广西植物研究所)
摘要:
为研究牛大力内生细菌多样性组成及其促生特性,从经表面消毒处理的牛大力根茎组织中分离内生细菌,采用16S rRNA序列分析法对内生细菌的多样性进行系统发育分析,并以固氮、溶磷、产铁载体、产IAA能力等为指标,对内生细菌进行促生活性检测。从牛大力根和茎中共分离到59株内生细菌,包括16属20种,芽孢杆菌属是牛大力的优势菌群;在20种分离菌株中,分别有11、17、7株具有潜在的固氮、溶磷、产铁载体的能力,有13株菌可以合成IAA。其中RH17-2、RH17-5、GR2-13株菌同时具备固氮、溶磷性、产铁载体、产IAA4种促生特性。从牛大力中分离得到的内生细菌具有丰富的物种多样性和促生特性,在未来的农业生产上具有潜在的应用价值。
关键词:  牛大力 内生细菌 多样性 促生特性 根茎 芽孢杆菌
DOI:
投稿时间:2020-02-18修订日期:2020-04-30
基金项目:广西重点研发计划项目(桂科AB16380210);广西创新驱动发展专项(桂科AA17204056)
Study on Diversity and Plant Growth Promoting Activities of the Endophytic Bacteria Isolated from Callerya speciose
sukun1, huangying1, liliangbo2, lidazhao3, tanghui4, huangrongshao1,2
(1.College of Agriculture, Guangxi University;2.Traditional Chinese Medicine, Guangxi University of Traditional Chinese Medicine;3.GUANGZE Health Industry Co., Ltd of Guangxi;4.Guangxi Institute of Botany, Chinese Academy of Sciences)
Abstract:
To explore the diversity composition of endophytic bacteria isolated from Callerya speciose. Endophytes were isolated from the plant tissues that were sterilized on the surface. The diversity of endophytic bacteria was chosen to make phylogenetic analysis for the genetic diversity by 16S rDNA sequence analysis, and the ability of nitrogen-fixation, phosphorus dissolution, siderophore production and IAA production were used as indicators to detect the viability of the representative trains. The results showed that 59 strains of endophytic bacteria , including 20 species of 16 genera, were isolated from the roots and stems of Callerya speciose, and Bacillus were dominant bacteria. Among the 20 strains, 11, 17 and 7strains had the potential of nitrogen-fixation phosphorus dissolution and siderophore production, and 13 strains could synthesize IAA. Among them, RH17-2, RH17-5 and GR2-1 strains had the characteristics of nitrogen fixation, phosphorus solubilization, siderophore production and IAA production. In summary, the endophytic bacteria isolated from Callerya speciose hawe rich diversity and growth promoting characteristics, and have potential application value in future agricultural producers.
Key words:  Study on Diversity and Plant Growth Promoting Activities of the Endophytic Bacteria Isolated from Callerya speciose

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