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唐健民,朱成豪,秦惠珍,唐启明,高丽梅,熊雅兰,蒋昊龙,韦记青.神木天坑两种药茶同源植物叶总黄酮提取工艺及抗氧化活性研究[J].广西科学院学报,2020,36(1):101-108. [点击复制]
- TANG Jianmin,ZHU Chenghao,QIN Huizhen,TANG Qiming,GAO Limei,XIONG Yalan,JIANG Haolong,WEI Jiqin.Study on Flavonoids Extraction and Antioxidant Activity of Two Herbal Tea Homologous Plants' Leaf in Shenmu Tiankeng[J].Journal of Guangxi Academy of Sciences,2020,36(1):101-108. [点击复制]
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| 神木天坑两种药茶同源植物叶总黄酮提取工艺及抗氧化活性研究 |
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唐健民, 朱成豪, 秦惠珍, 唐启明, 高丽梅, 熊雅兰, 蒋昊龙, 韦记青
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| (广西壮族自治区中国科学院广西植物研究所, 广西植物功能物质研究与利用重点实验室, 广西桂林 541006) |
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| 摘要: |
| 喀斯特天坑地区拥有许多特色的药茶资源。本研究采用超声波提取法对神木天坑五棱苦丁茶总黄酮进行提取，并运用单因素试验和响应面试验相结合的方式对提取条件进行优化。然后以神木天坑的五棱苦丁茶、异叶梁王茶的叶片为材料，以苦丁茶叶片为对照，测定3种药茶的总黄酮含量；同时，为研究3种药茶的抗氧化活性，进行体外羟基自由基（·OH）、超氧阴离子（·O2-）和二苯基苦基苯肼自由基（DPPH·）的清除试验。结果显示：乙醇浓度40%（V∶V）、料液比1∶50 （g/mL）、提取温度64.9℃、提取时间35.2 min、超声波功率316.8 W和超声频率35 kHz时，苦丁茶、五棱苦丁茶、异叶梁王茶总黄酮得率分别为10.61%、4.98%和15.73%。当控制黄酮浓度为1 mg/mL时，3种茶叶黄酮均具有较好的总还原力，抗氧化活性较好，其中以异叶梁王茶对·OH、·O2-和DPPH·的清除率最大，分别为53.02%、49.84%和73.96%。试验结果表明异叶梁王茶具有较好的抗氧化能力，为其资源开发奠定一定的科学基础。 |
| 关键词: 五棱苦丁茶 异叶梁王茶 总黄酮 响应面法 抗氧化 |
| DOI：10.13657/j.cnki.gxkxyxb.20200317.012 |
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| 基金项目:中央引导地方科技发展专项（桂科ZY1949013），广西科技基础和人才专项（桂科AD17129022），广西植物研究所基本业务费项目（桂植业18013，18014，19002）和广西植物功能物质研究与利用重点实验室项目（ZRJJ2018-9）资助。 |
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| Study on Flavonoids Extraction and Antioxidant Activity of Two Herbal Tea Homologous Plants' Leaf in Shenmu Tiankeng |
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TANG Jianmin, ZHU Chenghao, QIN Huizhen, TANG Qiming, GAO Limei, XIONG Yalan, JIANG Haolong, WEI Jiqin
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| (Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institure of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China) |
| Abstract: |
| The karst tiankeng area has many featured medicinal tea resources.In this study,ultrasonic extraction was used to extract the total flavonoids of Ilex pentagona,and the combination of single factor test and response surface test was used to optimize the extraction conditions.The leaves of Ilex pentagona and Nothopanax davidii in Shenmu Tiankeng area were used as test materials to determine the content of total flavonoids of three kinds of medicinal tea was measured with broadleaf holly leaf as a control.At the same time,in order to study the antioxidant activity of three kinds of medicinal tea,scavenging tests of hydroxyl radical (·OH),superoxide anion (·O2-) and diphenylpicryl hydrazine radical (DPPH·) were performed in vitro.The results showed that when the ethanol concentration was 40% (V:V),the solid-liquid ratio was 1:50 (g / mL),the extraction temperature was 64.9℃,the extraction time was 35.2 min,the ultrasonic power was 316.8 W,and the ultrasonic frequency was 35 kHz,the total flavonoid yields of broadleaf holly leaf,Ilex pentagona and Nothopanax davidii were 10.61%,4.98% and 15.73%,respectively.When the concentration of total flavonoids was controlled to 1 mg / mL,flavonoids of all three teas had better total reducing power and better antioxidant activity.Among them,the clearance rate of ·OH,·O2-,DPPH·by Nothopanax davidii was the largest,which were 53.02%,49.84%,and 73.96%,respectively.The test results show that Nothopanax davidi has good antioxidant capacity,which lays a certain scientific foundation for the development of its resources. |
| Key words: Ilex pentagona Nothopanax davidii total flavonoids response surface method antioxidant |
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