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胡艳玉,杨星,陈德斌,江泽权,黄小琼,吴强,黄荣韶,李良波.化州柚落花、落果及叶片中总黄酮含量及其抗氧化活性研究[J].广西科学院学报,2019,35(4):345-350. [点击复制]
- HU Yanyu,YANG Xing,CHEN Debin,JIANG Zequan,HUANG Xiaoqiong,WU Qiang,HUANG Rongshao,LI Liangbo.Study on Total Flavonoids and Antioxidant Activity in the Extract of Blossom Drop, Fruit Drop and Leaves of Citrus grandis ‘Tomentosa’[J].Journal of Guangxi Academy of Sciences,2019,35(4):345-350. [点击复制]
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| 化州柚落花、落果及叶片中总黄酮含量及其抗氧化活性研究 |
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胡艳玉1, 杨星1, 陈德斌2, 江泽权2, 黄小琼2, 吴强1, 黄荣韶1, 李良波1
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| (1.广西大学农学院, 广西南宁 530004;2.平果县天然润宝农业发展有限公司, 广西百色 531400) |
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| 摘要: |
| 本文以化州柚落花、落果及叶片为材料，研究60%乙醇提取和水提取两种方式所得提取液中总黄酮的含量及抗氧化能力。实验采用紫外分光光度法测定化州柚不同提取物中总黄酮的含量，并采用DPPH法和水杨酸法测定其抗氧化能力。结果表明，化州柚落果、落花及叶片的60%乙醇提取液总黄酮得率高于水提液；水提液对DPPH自由基的最大清除率均大于91%，60%乙醇提取液对DPPH自由基的清除率均高于88%，各提取液对DPPH自由基的清除率在一定范围内随反应时间及提取液浓度的增加而增高；落花60%乙醇提取液对羟基自由基的清除率远高于落果和叶片；羟基自由基的清除率与提取液浓度呈正相关关系，与反应时间无关。化州柚不同部位总黄酮含量相差很大，其含量变化为落果 > 落花 > 叶片，因此可根据总黄酮含量及抗氧化活性对化州柚落花、落果和叶进行分类利用，变废为宝。 |
| 关键词: 化州柚 浸提液 总黄酮 抗氧化性 DPPH自由基 |
| DOI：10.13657/j.cnki.gxkxyxb.20191210.003 |
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| 基金项目:百色市科技计划项目（百科20183006）资助。 |
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| Study on Total Flavonoids and Antioxidant Activity in the Extract of Blossom Drop, Fruit Drop and Leaves of Citrus grandis ‘Tomentosa’ |
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HU Yanyu1, YANG Xing1, CHEN Debin2, JIANG Zequan2, HUANG Xiaoqiong2, WU Qiang1, HUANG Rongshao1, LI Liangbo1
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| (1.College of Agriculture, Guangxi University, Nanning, Guangxi, 530004, China;2.TANGERINEBABY Agricultural Development Co., Ltd. of Pingguo County, Baise, Guangxi, 531400, China) |
| Abstract: |
| In this paper,Citrus grandis ‘Tomentosa’ flowers, fruits and leaves were used as materials to study the total flavonoid content and antioxidant capacity in the extracts obtained by 60% ethanol extraction and water extraction. UV spectrophotometry was used to determine the total flavonoids in different extracts of Citrus grandis ‘Tomentosa’, and DPPH and salicylic acid were used to determine its antioxidant capacity. The results showed that the yield of total flavonoids in 60% ethanol extract of Citrus grandis ‘Tomentosa’ blossom drops, fruits drops and leaves was higher than that of water extract. The maximum scavenging rate of DPPH free radicals by water extract was all greater than 91%. The scavenging rate of DPPH free radicals in 60% ethanol extracts was all higher than 88%. The scavenging rate of DPPH free radicals in each extract increased within a certain range as the reaction time and the concentration of the extract increased. The scavenging rate of hydroxyl radicals in 60% alcohol extract of blossom drops was much higher than that in fruit drops and leaves. The scavenging rate of hydroxyl radicals had a positive correlation with the concentration of the extract, and had nothing to do with the reaction time. The content of total flavonoids in different parts of Citrus grandis ‘Tomentosa’ is very different, and its content changes are fruit drop > blossom drop > leaf. Therefore, according to the total flavonoid content and antioxidant activity, the blossom drop, fruit drop and leaf of Citrus grandis ‘Tomentosa’ can be classified and used to turn waste into treasure. |
| Key words: Citrus grandis ‘Tomentosa’ extract total flavonoids antioxidant activity DPPH radical |
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