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荆圆圆,张天文,刘恩孚,陈群,孙铭,郭文,刘广斌.不同盐度下镉对太平洋牡蛎不同组织热休克蛋白70基因表达的影响[J].广西科学院学报,2019,35(4):332-336,344. [点击复制]
- JING Yuanyuan,ZHANG Tianwen,LIU Enfu,CHEN Qun,SUN Ming,GUO Wen,LIU Guangbin.Effects of Cadmium on Heat Shock Protein 70 Gene Expression in Different Tissues of Crassostrea gigasunder Different Salinity[J].Journal of Guangxi Academy of Sciences,2019,35(4):332-336,344. [点击复制]
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| 不同盐度下镉对太平洋牡蛎不同组织热休克蛋白70基因表达的影响 |
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荆圆圆1, 张天文1,2, 刘恩孚1, 陈群1, 孙铭1, 郭文1,2, 刘广斌1,3
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| (1.山东省海洋生物研究院, 山东青岛 266104;2.山东省海水健康养殖工程技术研究中心, 山东青岛 266104;3.山东省海水养殖病害防治重点实验室, 山东青岛 266104) |
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| 摘要: |
| 研究不同盐度作用下，镉对太平洋牡蛎（Crassostrea gigas）热休克蛋白70（Hsp70）基因表达的影响，为从养殖源头加强贝类的安全生产提供技术支撑，并为其产品质量控制提供基础数据。分别将太平洋牡蛎在盐度13 psu、20 psu、27 psu和34 psu下驯化14 d后，将其暴露于10 μg/L镉溶液中28 d进行蓄积实验，随后转移至干净的海水中进行排出实验；将未暴露于镉溶液的对应盐度处理组设置为空白组；最后采用实时荧光定量PCR法进行基因表达量测定。研究结果显示：4个盐度作用下，外套膜中Hsp70基因的mRNA表达趋势大体相同，体内镉浓度较低时Hsp70基因被诱导，镉浓度较高时Hsp70基因被抑制。消化腺Hsp70基因表达大部分受到抑制，盐度20 psu组于第42天受到诱导，盐度27 psu组于第28天受到诱导，盐度34 psu组于第7，21和42天受到诱导。表明Hsp70基因对重金属镉具有显著的反应性，作为镉早期污染综合预警指示分子具有可行性。 |
| 关键词: 太平洋牡蛎 镉 盐度 热休克蛋白70 基因表达 |
| DOI：10.13657/j.cnki.gxkxyxb.20191129.002 |
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| 基金项目:山东省农业重大应用技术创新课题"单体三倍体牡蛎生态化养殖模式构建与示范"项目和山东省现代农业产业技术体系贝类创新团队项目（STAID-14）资助。 |
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| Effects of Cadmium on Heat Shock Protein 70 Gene Expression in Different Tissues of Crassostrea gigasunder Different Salinity |
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JING Yuanyuan1, ZHANG Tianwen1,2, LIU Enfu1, CHEN Qun1, SUN Ming1, GUO Wen1,2, LIU Guangbin1,3
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| (1.Marine Biology Institute of Shandong Province, Qingdao, Shandong, 266104, China;2.Healthy Mariculture Engineering Research Center of Shandong Province, Qingdao, Shandong, 266104, China;3.Shandong Key Laboratory of Disease Control in Mariculture, Qingdao, Shandong, 266104, China) |
| Abstract: |
| The effect of cadmium on the expression of heat shock protein 70 (Hsp70) in Crassostrea gigas under different salinity was studied to provide technical support for strengthening the safe production of shellfish from breeding sources, and provide basic data for product quality control. Oysters were acclimatized to different salinities (13 psu, 20 psu, 27 psu, and 34 psu) for 14 d, and exposed to 10 μg/L Cd solution for 28 d for storage experiments, and then transferred to clean seawater for discharge experiment. The corresponding salinity treatment group not exposed to the cadmium solution was set as the blank group. Finally, the real-time fluorescence quantitative PCR method was used to determine the gene expression level. The results showed that the mRNA expression trend of Hsp70 gene in the mantle was generally the same under the effect of four salinities.Hsp70 gene was induced when the cadmium concentration in the body was low, and Hsp70 gene was suppressed when the cadmium concentration was high.Hsp70 expression in the digestive gland was mostly inhibited. The salinity 20 psu group was induced on day 42. The salinity 27 psu group was induced on day 28. The salinity 34 psu group was induced on days 7, 21, and 42. It shows that Hsp70 gene has significant reactivity to heavy metal cadmium, and it is feasible as a comprehensive early warning indicator of cadmium pollution. |
| Key words: Crassostrea gigas cadmium salinity heat shock protein 70 gene expression |
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